ISSN:
0741-0581
Keywords:
GACH
;
Amino-resin
;
SEM
;
Preparation
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
Biological specimens can be prepared for scanning electron microscopy by means of copolymerizing the fixing agent glutaraldehyde with carbohydrazide prior to air drying. Such preparations are more stable in the electron microscope, show less internal cellular disruption and retain more of their native elemental composition than specimens prepared by means of dehydration and critical-point drying. Specimens observed in the scanning electron microscope can often be recovered for thin sectioning with no additional embedment, and can then be observed by means of transmission elecltron microscopy. The preparation (termed GACH) can be performed in almost any laboratory with no specialized equipment and, for the most part, may be carried out at room temperature. The technique appears to provide the promise of further research applications in scanning electron microscopy which may employ conjugated procedures of immunocytochemistry and cathodoluminescence as well as X-ray microanalysis in limited situations.
Additional Material:
14 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060010203
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