Publication Date:
1984-10-19
Description:
The techniques used for the characterization of protein and peptide structure have undergone great changes that have improved the speed, reliability, and applicability of the process. High-performance liquid chromatography and gel electrophoresis have made the purification of proteins and peptides a routine procedure, even when the compound of interest is a minor component of a complex biological mixture. The chemistry and instrumentation used in amino acid analysis and amino acid sequencing now permit the analysis of as little as 5 to 50 picomoles of samples. This represents an increase in sensitivity of more than a thousandfold over the last 10 years and has made possible the structural analysis of a wide variety of scarce but important compounds.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hunkapiller, M W -- Strickler, J E -- Wilson, K J -- New York, N.Y. -- Science. 1984 Oct 19;226(4672):304-11.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6385254" target="_blank"〉PubMed〈/a〉
Keywords:
*Amino Acid Sequence
;
Amino Acids/analysis
;
Chemical Fractionation
;
Chemistry Techniques, Analytical/*methods
;
Chromatography
;
Chromatography, High Pressure Liquid
;
Chromatography, Ion Exchange
;
Electrophoresis, Polyacrylamide Gel
;
Isoelectric Focusing
;
Peptide Hydrolases
;
Peptides/analysis
;
Proteins/*analysis/isolation & purification
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
