Publication Date:
2012-04-17
Description:
As with many other viruses, the initial cell attachment of rotaviruses, which are the major causative agent of infantile gastroenteritis, is mediated by interactions with specific cellular glycans. The distally located VP8* domain of the rotavirus spike protein VP4 (ref. 5) mediates such interactions. The existing paradigm is that 'sialidase-sensitive' animal rotavirus strains bind to glycans with terminal sialic acid (Sia), whereas 'sialidase-insensitive' human rotavirus strains bind to glycans with internal Sia such as GM1 (ref. 3). Although the involvement of Sia in the animal strains is firmly supported by crystallographic studies, it is not yet known how VP8* of human rotaviruses interacts with Sia and whether their cell attachment necessarily involves sialoglycans. Here we show that VP8* of a human rotavirus strain specifically recognizes A-type histo-blood group antigen (HBGA) using a glycan array screen comprised of 511 glycans, and that virus infectivity in HT-29 cells is abrogated by anti-A-type antibodies as well as significantly enhanced in Chinese hamster ovary cells genetically modified to express the A-type HBGA, providing a novel paradigm for initial cell attachment of human rotavirus. HBGAs are genetically determined glycoconjugates present in mucosal secretions, epithelia and on red blood cells, and are recognized as susceptibility and cell attachment factors for gastric pathogens like Helicobacter pylori and noroviruses. Our crystallographic studies show that the A-type HBGA binds to the human rotavirus VP8* at the same location as the Sia in the VP8* of animal rotavirus, and suggest how subtle changes within the same structural framework allow for such receptor switching. These results raise the possibility that host susceptibility to specific human rotavirus strains and pathogenesis are influenced by genetically controlled expression of different HBGAs among the world's population.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3350622/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3350622/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hu, Liya -- Crawford, Sue E -- Czako, Rita -- Cortes-Penfield, Nicolas W -- Smith, David F -- Le Pendu, Jacques -- Estes, Mary K -- Prasad, B V Venkataram -- AI 080656/AI/NIAID NIH HHS/ -- AI36040/AI/NIAID NIH HHS/ -- GM62116/GM/NIGMS NIH HHS/ -- P30 DK056338/DK/NIDDK NIH HHS/ -- P30 DK56338/DK/NIDDK NIH HHS/ -- P41 GM103694/GM/NIGMS NIH HHS/ -- R01 AI080656/AI/NIAID NIH HHS/ -- U54 GM062116/GM/NIGMS NIH HHS/ -- U54 GM062116-01A1/GM/NIGMS NIH HHS/ -- England -- Nature. 2012 Apr 15;485(7397):256-9. doi: 10.1038/nature10996.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22504179" target="_blank"〉PubMed〈/a〉
Keywords:
ABO Blood-Group System/chemistry/genetics/immunology/*metabolism
;
Amino Acid Sequence
;
Animals
;
CHO Cells
;
Cricetinae
;
Crystallography, X-Ray
;
Erythrocytes/metabolism/virology
;
Host Specificity/*physiology
;
Humans
;
Models, Molecular
;
Molecular Sequence Data
;
N-Acetylneuraminic Acid/antagonists & inhibitors/chemistry/immunology/metabolism
;
RNA-Binding Proteins/chemistry/*metabolism
;
Receptors, Virus/chemistry/genetics/*metabolism
;
*Rotavirus/chemistry/classification/metabolism/pathogenicity
;
Viral Nonstructural Proteins/chemistry/*metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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