Publication Date:
2005-06-11
Description:
In Schizosaccharomyces pombe, the RNA interference (RNAi) machinery converts pericentromeric transcripts into small interfering RNAs (siRNAs) and is required for the assembly of pericentromeric heterochromatin. Here we describe a mutation in the second largest subunit of RNA polymerase II (RNAPII). Both wild-type and mutant RNAPII localized to the pericentromere. However, the mutation resulted in the loss of heterochromatic histone modifications and in the accumulation of pericentromeric transcripts, accompanied by the loss of siRNAs. This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kato, Hiroaki -- Goto, Derek B -- Martienssen, Robert A -- Urano, Takeshi -- Furukawa, Koichi -- Murakami, Yota -- R01-GM067014/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2005 Jul 15;309(5733):467-9. Epub 2005 Jun 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15947136" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Centromere/metabolism
;
Chromosome Segregation
;
Gene Expression Regulation, Fungal
;
Heterochromatin/*metabolism
;
Histones/metabolism
;
Methylation
;
Molecular Sequence Data
;
Oligonucleotide Array Sequence Analysis
;
Point Mutation
;
*RNA Interference
;
RNA Polymerase II/chemistry/genetics/*metabolism
;
RNA, Fungal/metabolism
;
RNA, Messenger/metabolism
;
RNA, Small Interfering/*metabolism
;
Schizosaccharomyces/enzymology/genetics/*metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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