Publication Date:
2001-02-13
Description:
We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing alpha-kinase domain was functional. Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Runnels, L W -- Yue, L -- Clapham, D E -- New York, N.Y. -- Science. 2001 Feb 9;291(5506):1043-7. Epub 2001 Jan 18.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Cardiology, Department of Neurobiology, Harvard Medical School, 1309 Enders Building, 320 Longwood Avenue, Children's Hospital, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11161216" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/metabolism
;
Amino Acid Motifs
;
Amino Acid Sequence
;
Animals
;
CHO Cells
;
Calcium/metabolism
;
Catalytic Domain
;
Cations/metabolism
;
Cell Line
;
Cricetinae
;
DNA, Complementary
;
Electric Conductivity
;
Humans
;
Ion Channels/chemistry/*genetics/*metabolism
;
*Membrane Proteins
;
Mice
;
Molecular Sequence Data
;
Mutation
;
Myelin Basic Protein/metabolism
;
Patch-Clamp Techniques
;
Phosphorylation
;
Protein Kinases/chemistry/*genetics/*metabolism
;
Protein-Serine-Threonine Kinases
;
Rats
;
Recombinant Fusion Proteins/chemistry/metabolism
;
TRPM Cation Channels
;
Transfection
;
Two-Hybrid System Techniques
;
Type C Phospholipases/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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