ISSN:
1617-4623
Keywords:
Brassica oleracea
;
Self-incompatibility
;
Pulsed-field gel electrophoresis
;
Allelic variability
;
S locus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary In Brassica oleracea, the pollen-stigma interaction of self-incompatibility is controlled by a single genetically defined locus designated S. Molecular studies have identified two genes that are tightly linked to the classically defined S locus: The S-Locus Glycoprotein (SLG) gene and the S-Receptor Kinase (SRK) gene. In previous RFLP linkage analyses with probes specific for SLG and SRK, we were unable to identify any recombination events between SLG, SRK, and self-incompatibility phenotype. In this paper, we use pulsed-field gel electrophoresis (PFGE) in conjunction with DNA blot analysis to characterize the S-locus region from two highly divergent self-incompatibility genotypes, S 2 and S 6. We establish the physical linkage of SLG and SRK in each genotype, and demonstrate that the two genes are separated by a maximum distance of 220 kb in the S 6 genotype and 350 kb in the S 2 genotype. Furthermore, a comparison of the data from the two genotypes reveals that a high level of polymorphism exists across the entire S-locus region.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00277135
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