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  • 1
    Electronic Resource
    Electronic Resource
    The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinipis alba L. are localized in the exine cell wall layer of developing microspores (1994)
    Springer
    Planta 192 (1994), S. 221-231 
    Details
    ISSN: 1432-2048
    Keywords: Exine ; Flower-specific cDNAs ; Maltose-binding protein fusions ; Peritapetal membrane ; Pro-Ubisch bodies ; Signal sequence ; Sinapis ; Tapetum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By differential screening of a copy DNA (cDNA) library from flowering Sinapis alba L. apices against cDNAs from vegetative apices, two cDNA clones were isolated representing transcripts that are expressed transiently at an early stage of tapetum development. The Satap35 cDNA encodes a polypeptide with a predicted molecular weight of 12.7 kDa and an isoelectric point of 10.4. The Satap44 cDNA codes for a putative 12.4-kDa polypeptide with an isoelectric point of 7.5. The deduced amino-acid sequences display 76% sequence identity and contain an N-terminal stretch of hydrophobic amino acids which has characteristics of secretory signal sequences. In-vitro transcription of the cDNAs and translation of the resulting RNAs in the presence of canine pancreatic microsomes demonstrates that the two proteins are translocated into the microsomes and that the putative preproteins are proteolytically processed to the mature forms. By immunoelectron microscopy the SaTAP35 and SaTAP44 proteins were detected at the developing peritapetal membrane between the tapetal cytoplasm and the adjacent middle layer of the anther wall. Furthermore, labelling was observed within the locule in association with globules resembling pro-Ubisch bodies which appeared at the tetrad stage. During the early vacuolate stage of microspore development the young exine was strongly labelled. The exine and the peritapetal membrane both are composed of sporopollenin, and the pro-Ubisch bodies are thought to contain sporopollenin precursors. Thus, SaTAP35 and SaTAP44 might be involved in sporopollenin formation and/or deposition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194456
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  • 2
    Electronic Resource
    Electronic Resource
    Transformation of the developing barley endosperm by particle bombardment (1991)
    Springer
    Planta 185 (1991), S. 330-336 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone ; Endosperm, starchy ; Gene expression, transient ; β-Glucuronidase ; Hordein ; Hordeum (endosperm transformation) ; Particle bombardment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Delivery of DNA into intact cells of the developing barley (Hordeum vulgare L.) endosperm was performed with the BIOLISTIC particle gun. It is shown that the proximal 532 base pairs (bp) of the upstream region of a B1-hordein gene drive the expression of the β-glucuronidase (GUS) gene (uidA) in sub-aleurone and starchy-endosperm cells but not in cells devoid of starch, i.e. developing aleurone cells. The 35S promoter from cauliflower mosaic virus was active in all three cell types. This cell-specific activity of the hordein promoter was verified by a detailed histological study of the regions of the extruded endosperms expressing the uidA gene. The analysis included a histological study of the developing endosperm as a base for classifying the different cell types in the developing endosperm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201052
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  • 3
    Electronic Resource
    Electronic Resource
    The proteins encoded by two tapetum-specific transcripts,Satap35 andSatap44, fromSinapis alba L. are localized in the exine cell wall layer of developing microspores (1994)
    Springer
    Planta 192 (1994), S. 221-231 
    Details
    ISSN: 1432-2048
    Keywords: Exine ; Flower-specific cDNAs ; Maltose-binding protein fusions ; Peritapetal membrane ; Pro-Ubisch bodies ; Signal sequence ; Sinapis ; Tapetum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By differential screening of a copy DNA (cDNA) library from floweringSinapis alba L. apices against cDNAs from vegetative apices, two cDNA clones were isolated representing transcripts that are expressed transiently at an early stage of tapetum development. TheSatap35 cDNA encodes a polypeptide with a predicted molecular weight of 12.7 kDa and an isoelectric point of 10.4. TheSatap44 cDNA codes for a putative 12.4-kDa polypeptide with an isoelectric point of 7.5. The deduced amino-acid sequences display 76% sequence identity and contain an N-terminal stretch of hydrophobic amino acids which has characteristics of secretory signal sequences. In-vitro transcription of the cDNAs and translation of the resulting RNAs in the presence of canine pancreatic microsomes demonstrates that the two proteins are translocated into the microsomes and that the putative preproteins are proteolytically processed to the mature forms. By immunoelectron microscopy theSaTAP35 andSaTAP44 proteins were detected at the developing peritapetal membrane between the tapetal cytoplasm and the adjacent middle layer of the anther wall. Furthermore, labelling was observed within the locule in association with globules resembling pro-Ubisch bodies which appeared at the tetrad stage. During the early vacuolate stage of microspore development the young exine was strongly labelled. The exine and the peritapetal membrane both are composed of sporopollenin, and the pro-Ubisch bodies are thought to contain sporopollenin precursors. Thus,SaTAP35 andSaTAP44 might be involved in sporopollenin formation and/or deposition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01089038
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