Publication Date:
1996-08-30
Description:
The immediate-early transcription factor NGFI-A (also called Egr-1, zif/268, or Krox-24) is thought to couple extracellular signals to changes in gene expression. Although activins and inhibins regulate follicle-stimulating hormone (FSH) synthesis, no factor has been identified that exclusively regulates luteinizing hormone (LH) synthesis. An analysis of NGFI-A-deficient mice derived from embryonic stem cells demonstrated female infertility that was secondary to LH-beta deficiency. Ovariectomy led to increased amounts of FSH-beta but not LH-beta messenger RNA, which suggested a pituitary defect. A conserved, canonical NGFI-A site in the LH-beta promoter was required for synergistic activation by NGFI-A and steroidogenic factor-1 (SF-1). NGFI-A apparently influences female reproductive capacity through its regulation of LH-beta transcription.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lee, S L -- Sadovsky, Y -- Swirnoff, A H -- Polish, J A -- Goda, P -- Gavrilina, G -- Milbrandt, J -- CA53524/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1996 Aug 30;273(5279):1219-21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8703054" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
Cell Line
;
DNA-Binding Proteins/*genetics
;
Early Growth Response Protein 1
;
Female
;
Follicle Stimulating Hormone/genetics
;
Follicle Stimulating Hormone, beta Subunit
;
Fushi Tarazu Transcription Factors
;
*Gene Expression Regulation
;
Gene Targeting
;
Gonadotropins/pharmacology
;
Homeodomain Proteins
;
*Immediate-Early Proteins
;
Infertility, Female/*genetics
;
Luteinizing Hormone/analysis/*deficiency/*genetics
;
Male
;
Mice
;
Molecular Sequence Data
;
Ovary/drug effects/physiology
;
Pituitary Gland/metabolism
;
Promoter Regions, Genetic
;
Receptors, Cytoplasmic and Nuclear
;
Steroidogenic Factor 1
;
Transcription Factors/*genetics
;
Transfection
;
Uterus/drug effects
;
Zinc Fingers
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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