ISSN:
1617-4623
Schlagwort(e):
A. tumefaciens
;
Agroinfection, virulence genes
;
T-DNA transfer
;
Cauliflower mosaic virus
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary To test whether virulence mutants of Agrobacterium tumefaciens are capable of promoting T-DNA transfer into plant cells, a tandem array of Cauliflower Mosaic Virus (CaMV) DNA was cloned between T-region border sequences on a wide host range plasmid and introduced into various virulence mutants. The resulting strains were used to infect Brassica rapa cv. Just Right. This assay, recently referred to as agroinfection, is based on the appearance of viral symptoms following transfer of T-DNA to plant cells, and is shown to be at least 100 times more sensitive in detecting T-DNA transfer than tumour formation. Mutants in the loci vir A, B and G, which were avirulent on turnip, failed to induce virus symptoms. Of the two vir D mutants tested, neither induced tumours, but one was capable of inducing virus symptoms. Mutants in vir E, C and F, which induced respectively no, small and normal tumours on turnip, all induced virus symptoms.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00338075
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