ISSN:
1432-203X
Keywords:
Key words Transgenic peanut
;
Agrobacterium
;
Transformation
;
Transgene expression
;
Transgene inheritance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract To evaluate and characterize the stability of traits transferred via Agrobacterium transformation, foreign gene expression must be examined in sexually derived progeny. The objective of this study was to analyze three transgenic peanut plants, 1-10, 12-1, and 17-1, for the inheritance and expression of their foreign genes. Segregation ratios for the introduced genes in T2 plants gave either 100% or 3:1 expression of the β-glucuronidase (GUS) gene, demonstrating recovery of both homozygous and heterozygous T1 plants. Fluorometric GUS assay in T1 and T2 generations of all three plants showed that the GUS gene was stably expressed in the progeny. DNA analyses showed 100% concordance between the presence of the foreign gene and enzyme activity. Our results demonstrate that transgenes in peanut introduced by Agrobacterium can be inherited in a Mendelian manner.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01142320
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