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  • Age determination  (23)
  • LIFE SCIENCES (GENERAL)
  • 1
    Publication Date: 2019-07-13
    Description: Experiments performed on the heat resistant organism CK 4-6 are described. Its response to dry heat at two temperatures (125 C and 135 C) at eight humidity levels (0.001 percent to 100 percent RH) in a closed can system is studied.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-CR-149658 , QPR-46
    Format: application/pdf
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  • 2
    Publication Date: 2019-07-13
    Description: A series of experiments was conducted to determine the dry heat resistance of microorganisms in soil obtained from Denver Colorado, Pasadena California, Kennedy Space Center Florida, and Cincinnati Ohio. The results of the KSC terminal sterilization cycle experiment are given in graphs. The average number of viable organisms per m1 was calculated for 18 replicate soil samples for each sample area and points plotted equivalent to 30 hr exposure at 112 C. The result showed a reduction of 3 logs from the initial population for both KSC and Cincinnati soil samples. Results from other areas are given in graphs.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-CR-142909 , QPR-39
    Format: application/pdf
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  • 3
    Publication Date: 2019-07-13
    Description: Dry heat inactivation characteristics were compared for 4-6 (B. brevis) spores and microbes from the Cincinnati soil samples at 105, 112, and 125. Characterized were the survival curves of 4-6 (B. brevis) spores at 112, 115, 118, 120, and 125 C, and 1.2 microgram of water per ml of headspace air (closed tin-can system), and the morphological characteristics of 4-6 (B. brevis), 6-12 (B. lentus), 7-11 (B. coagulans), and B. subtilis var. niger spores by scanning electron microscopy.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-CR-142296 , QPR-38
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  • 4
    Publication Date: 2019-07-13
    Description: Dry heat treatment is specified as the preferred means for the terminal sterilization of spacecraft and for decontamination of spacecraft components. The presence of organisms highly resistant to dry heat in soil and fallout around assembly and industrial manufacturing areas is shown. The dry heat survival characteristics of the Cape Kennedy isolate 4-6 B. brevis spores is demonstrated. The presence of hardy organisms from soil samples obtained from geographical areas of the United States is shown. A resistant fraction appears to occur in low numbers in a soil sample. The heat resistance characteristics of 4-6 B. brevis and B. subtilis var. niger spores are compared. Their morphological characteristics are compared by scanning electron microscopy.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-CR-145480 , QPR-40
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  • 5
    Publication Date: 2019-07-13
    Description: The experiments conducted to determine the heat resistance of Bacillus megaterium ATCC 6458 at 90 and 100 C were completed. Estimates from replicate experiments at eight percent relative humidities (less than 0.001 to 100% RH) for each temperature were computed. A Bacillus cereus strain with high heat resistance was cultured and the resistance determined in phosphate buffer (D sub 121.1 = 2.16 min and z = 8.7 C). The profile of the dry heat resistance of B. megaterium is summarized and the most resistant condition to the three spores (Bacillus subtilis var. niger, ATCC 29669, and Bacillus stearothermophilus, strain 1518) is compared.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-CR-158627 , QPR-54
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  • 6
    Publication Date: 2019-07-13
    Description: Statistical techniques which have relevance to studies on the thermal inactivation on bacterial spores are discussed.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-CR-146429 , QPR-42
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  • 7
    Publication Date: 2019-06-27
    Description: Dry heat sterilization of spacecraft was investigated by studying the production of spore crops, and thermal inactivation of the spores, and bacillus subtillus. Spore assays were made by conventional plate count methods, and survival curves for the spores are presented. The results indicate that the inherent resistance of spores from a parent cell can be maintained.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-CR-141122 , QPR-37
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  • 8
    Publication Date: 2019-07-13
    Description: The heat resistance of Bacillus subtilis var. niger was measured from 85 to 125 C using moisture levels of % RH or = 0.001 to 100. Curves are presented which characterize thermal destruction using thermal death times defined as F values at a given combination of three moisture and temperature conditions. The times required at 100 C for reductions of 99.99% of the initial population were estimated for the three moisture conditions. The linear model (from which estimates of D are obtained) was satisfactory for estimating thermal death times (% RH or = 0.07) in the plate count range. Estimates based on observed thermal death times and D values for % RH = 100 diverged so that D values generally gave a more conservative estimate over the temperature range 90 to 125 C. Estimates of Z sub F and Z sub L ranged from 32.1 to 58.3 C for % RH of or = 0.07 and 100. A Z sub D = 30.0 was obtained for data observed at % RH or = 0.07.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-CR-146549 , QPR-43
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  • 9
    Publication Date: 2019-07-13
    Description: Spores of Bacillus subtilis var. niger were heat treated in aqueous suspension at 90 C, and observed for morphological changes and loss of viability. The 5 logs reduction that occurred in broth at 90 min required 210 min in buffered water. Five characteristic changes observed after spores were exposed 120 min at 90 C in buffered water were: (1) 90% loss of spore viability, (2) 5% stainability, (3) 76% increase in spore size (as observed by scanning electron microscopy), (4) 21% of spore areas remaining refractile, and (5) an increase of 77% in packed cell volume (PCV). Stainability and PCV changes were recognized only after secondary exposure in broth. Extended heat exposure (3 h at 90 C) resulted in 99% loss of spore viability and 99% loss of stainability. After 4 hours of heat exposure, 90% of the cells disintegrated. These results suggest that early germinal changes occurr concurrently with the early changes in the heat susceptibility of dormant spores.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-CR-146401 , QPR-41
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  • 10
    Publication Date: 2021-05-19
    Description: Se entregan los resultados obtenidos de las entrevistas a los pescadores artesanales; cruceros de pesca de investigación y estaciones bio-oceanográficas y muestreos biológicos de la captura artesanal en los principales centros de actividades de pescadores artesanales de merluza del sur.
    Description: This paper gives the results obtained from interviews with fishermen, fishing cruises research and bio-oceanographic stations and biological sampling of the artisanal catch in the main centers of artisanal fishing activities of southern hake.
    Description: Unpublished
    Keywords: Demersal fish ; Biomass ; Bathymetric data ; Recruitment ; Spawning ; Stock assessment ; Age determination ; Size-at-age ; Length-weight relationships ; Salinity ; Sexual maturity ; Biological data ; Landing statistics
    Repository Name: AquaDocs
    Type: Report , Refereed , Article
    Format: 145pp. & Tablas
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