ISSN:
1573-5044
Keywords:
Agave tequilana
;
gelrite
;
glutamate dehydrogenase
;
vitrification
;
water potential
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Agave tequilana stem explants were used to produce adventitious shoots under a set of different water potentials induced by different concentrations of gelrite in the medium. At high water potentials all shoots were vitrified; as the medium water potential became more negative the degree of vitrification decreased but the number of shoots per explant also diminished. The enzymes NADH and NAD-GDH (EC. 1.4.1.2) were measured along the water potential gradient. GDH activity was high in the non-vitrified tissues and decreased significantly in the vitrified ones.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00043690
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