ISSN:
1573-0603
Keywords:
Adhesin
;
Antibody
;
Bacteria
;
Fab
;
Phage display
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Surface proteins provide a multitude of functions for the bacterial cell. Antibodies to these proteins can provide tools for tagging bacteria and characterizing protein function. Phage display technology has emerged as a powerful method for producing monoclonal Fabs in Escherichia coli. In an effort to study the adhesion mechanisms of Streptococcus parasanguis FW213, Fabs specific for the surface adhesin protein Fap1 were produced using phage display. The immune repertoire of a mouse injected with purified Fap1 was cloned into the phagemid vector pCOMB3, and a combinatorial Fab library was expressed in E. coli. A cell-based panning method using whole S. parasanguis cells was developed and has been shown to be a means for enriching for Fabs specific for the Fap1 protein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009814203184
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