Publication Date:
1993-10-01
Description:
In the assembly of a prespliceosome, U2 small nuclear ribonucleoprotein (snRNP) functions in pre-messenger RNA (mRNA) splicing together with splicing factors (SFs) 3a, SF3b, and several other proteins. The 17S but not the 12S form of U2 snRNP is active in splicing-complex formation. Here it is shown that the SF3a subunits correspond to three of the 17S U2 snRNP-specific polypeptides. SF3a interacts with U2 snRNP in the presence of SF3b to generate a structure similar to 17S U2 snRNP, which suggests a function for SF3a and SF3b in the incorporation of U2 snRNP into the spliceosome. Furthermore, the 60-kilodalton subunit of SF3a is related to the yeast splicing protein PRP9.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Brosi, R -- Groning, K -- Behrens, S E -- Luhrmann, R -- Kramer, A -- New York, N.Y. -- Science. 1993 Oct 1;262(5130):102-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departement de Biologie Cellulaire, Sciences III, Universite de Geneve, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8211112" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/metabolism
;
Cross Reactions
;
Electrophoresis, Polyacrylamide Gel
;
Fungal Proteins/chemistry/immunology/*metabolism
;
Humans
;
Nuclear Proteins/chemistry/immunology/*metabolism
;
*RNA Splicing
;
RNA-Binding Proteins
;
Ribonucleoprotein, U2 Small Nuclear/chemistry/*metabolism
;
*Saccharomyces cerevisiae Proteins
;
Spliceosomes/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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