ISSN:
1432-2048
Keywords:
Adenosine 5′-phosphosulfate
;
Catharanthus
;
Sulfate reduction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cell homogenates from Catharanthus roseus (L.) G. Don. grown S-autotrophically on sulfate in the dark are capable of reducing adenylysulfate (APS) to cysteine. This reduction required a particulate protein fraction from the cell extract and reduced ferredoxin as the electron donor. The protein fraction (MW 700,000±50,000) was found to contain Fd:NADP+ reductase, glutathione reductase and an unspecific dithiol reductase, and APS-sulfotransferase and thiosulfonate reductase activity. Resolution into these individual enzyme activities led to a non-restorable loss of the APS reducing activity. It was observed that a slow gradual decay of the APS reducing activity was accompanied by a likewise slow generation of a ferredoxin-dependent sulfite reductase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392546
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