Publication Date:
2001-08-11
Description:
Dynamic control of interferon-beta (IFN-beta) gene expression requires the regulated assembly and disassembly of the enhanceosome, a higher-order nucleoprotein complex formed in response to virus infection. The enhanceosome activates transcription by recruiting the histone acetyltransferase proteins CREB binding protein (CBP) and p300/CBP-associated factors (PCAF)/GCN5, which, in addition to modifying histones, acetylate HMGI(Y), the architectural component required for enhanceosome assembly. We show that the accurate execution of the IFN-beta transcriptional switch depends on the ordered acetylation of the high-mobility group I protein HMGI(Y) by PCAF/GCN5 and CBP, which acetylate HMGI(Y) at distinct lysine residues on endogenous promoters. Whereas acetylation of HMGI(Y) by CBP at lysine-65 destabilizes the enhanceosome, acetylation of HMGI(Y) by PCAF/GCN5 at lysine-71 potentiates transcription by stabilizing the enhanceosome and preventing acetylation by CBP.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Munshi, N -- Agalioti, T -- Lomvardas, S -- Merika, M -- Chen, G -- Thanos, D -- 1RO1GM54605/GM/NIGMS NIH HHS/ -- 5-T32-GM07367/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Aug 10;293(5532):1133-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11498590" target="_blank"〉PubMed〈/a〉
Keywords:
Acetylation
;
Acetyltransferases/metabolism
;
Amino Acid Sequence
;
CREB-Binding Protein
;
Cell Cycle Proteins
;
*Enhancer Elements, Genetic
;
*Gene Expression Regulation
;
HMGA1a Protein
;
HeLa Cells
;
High Mobility Group Proteins/chemistry/*metabolism
;
Histone Acetyltransferases
;
Histones/metabolism
;
Humans
;
Interferon-beta/*genetics
;
Lysine/metabolism
;
Molecular Sequence Data
;
Mutation
;
Nuclear Proteins/metabolism
;
Promoter Regions, Genetic
;
Protein Binding
;
Recombinant Proteins/metabolism
;
Respirovirus/physiology
;
*Saccharomyces cerevisiae Proteins
;
Trans-Activators/metabolism
;
Transcription Factors/chemistry/*metabolism
;
*Transcriptional Activation
;
Transfection
;
p300-CBP Transcription Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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