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  • Articles  (2)
  • Acetylene reduction assay  (2)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (2)
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  • Articles  (2)
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  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (2)
  • Geosciences  (2)
  • Biology  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 13 (1992), S. 173-175 
    ISSN: 1432-0789
    Keywords: Methidathion ; Soil microflora ; Enumeration of functional bacterial groups ; Acetylene reduction assay ; ARA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We studied the effects of the organophosphorus insecticide methidathion, at concentrations of 10, 50, 100, 200 and 300 μg g-1 in an agricultural soil, on fungi, total bacterial populations, aerobic N2-fixing bacteria, denitrifying bacteria, nitrifying bacteria (phases I and II), and nitrogenase activity (acetylene reduction assay). The presence of 10–300 μg g-1 of methidathion significantly increased fungal populations (colony-forming units). Denitrifying bacteria, aerobic N2-fixing bacteria and N2 fixation were significantly increased at concentrations of 50–300 μg g-1. The total number of bacteria increased significantly at concentrations of 100–300 μg g-1. Nitrifying bacteria decreased initially at concentrations of 300 μg g-1, but recovered rapidly to levels similar to those in the control soil without the insecticide.
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  • 2
    ISSN: 1432-0789
    Keywords: A. chroococcum ; Seed inoculation ; Zea mays ; Acetylene reduction assay ; Yield response ; Associative N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inoculated and non-inoculated seedlings of Zea mays were grown in agricultural soils under aseptic and non-sterile conditions. Acetylene reduction activity and microbial counts were determined after 7 and 30 days of growth. Irrespective of the soil type Azotobacter spp. were commonly isolated under maize cultivation. Inoculation of agricultural soils with a suspension of A. chroococcum led to an increase in Azotobacter numbers, although this effect diminished with time. Nitrogenase activity was detected on maize roots and increased in response to the inoculation with A. chroococcum, showing that this associative growth could be of primary importance for the plant. The results of assays for acetylene reduction activity indicated that the nitrogenase activity was associated only with the root systems.
    Type of Medium: Electronic Resource
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