Publication Date:
2008-07-26
Description:
Replicated chromosomes are held together by the chromosomal cohesin complex from the time of their synthesis in S phase onward. This requires the replication fork-associated acetyl transferase Eco1, but Eco1's mechanism of action is not known. We identified spontaneous suppressors of the thermosensitive eco1-1 allele in budding yeast. An acetylation-mimicking mutation of a conserved lysine in cohesin's Smc3 subunit makes Eco1 dispensable for cell growth, and we show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion. A second set of eco1-1 suppressors inactivate the budding yeast ortholog of the cohesin destabilizer Wapl. Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rolef Ben-Shahar, Tom -- Heeger, Sebastian -- Lehane, Chris -- East, Philip -- Flynn, Helen -- Skehel, Mark -- Uhlmann, Frank -- New York, N.Y. -- Science. 2008 Jul 25;321(5888):563-6. doi: 10.1126/science.1157774.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln'sInn Fields, London WC2A 3PX, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18653893" target="_blank"〉PubMed〈/a〉
Keywords:
Acetylation
;
Acetyltransferases/chemistry/genetics/*metabolism
;
Alleles
;
Cell Cycle Proteins/chemistry/genetics/*metabolism
;
Chondroitin Sulfate Proteoglycans/chemistry/genetics/*metabolism
;
Chromatids/*physiology
;
Chromosomal Proteins, Non-Histone/chemistry/genetics/*metabolism
;
Chromosomes, Fungal/*physiology
;
DNA Repair
;
DNA Replication
;
DNA, Fungal/metabolism
;
Mutation
;
Nuclear Proteins/chemistry/genetics/*metabolism
;
Protein Subunits/chemistry/genetics/metabolism
;
S Phase
;
Saccharomyces cerevisiae/genetics/growth & development/*physiology
;
Saccharomyces cerevisiae Proteins/chemistry/genetics/*metabolism
;
Suppression, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
