ISSN:
1615-6102
Keywords:
Abscission
;
β 1,4 glucan-4-glucanohydrolase
;
Cellulase localization
;
Ethylene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Antibodies raised against 9.5 cellulase (β 1,4 glucan-4-glucanohydrolase) were used to investigate the distribution of the enzyme in abscising bean petioles (Phaseolus vulgaris L. cv. Red Kidney). Results showed the enzyme to be localized in the cortical separation layer, which is 2 or 3 cells wide; this distribution is consistent with the proposed role of cellulase in abscission. Unfortunately, the enzyme's distribution in the stele was masked by non-specific staining. Direct assay of small fragments dissected from the abscission zone confirmed these findings and also showed very high activity levels in the stele. The vascular cellulase spread acropetally from the separation zone some 4 mm into the pulvinus. Experiments isolating the distal pulvinus prior to induction of abscission revealed that this activity was not the result of diffusion of cellulase from the separation layer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01287451
Permalink
