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  • 1
    Electronic Resource
    Electronic Resource
    Stress-related levels of abscisic acid in guard cell protoplasts of Vicia faba L. (1982)
    Springer
    Planta 154 (1982), S. 24-28 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Guard cell protoplast ; Protoplast (guard cells) ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Levels of abscisis acid (ABA) were determined in isolated guard cell (GCP) and mesophyll cell (MCP) protoplasts of Vicia faba L. in relation to water stress. Incubation of GCP and MCP in 0.4 M or 0.8 M mannitol resulted in an average increase in the level of free abscisic acid (ABA) in the cells of 34% (GCP) and 38% (MCP) within 15–60 min. It is concluded that guard cell protoplasts form ABA in response to osmotic stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385492
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on isolated starch-containing (Vicia faba) and starch-deficient (Allium cepa) guard cell protoplasts (1978)
    Springer
    Planta 143 (1978), S. 33-39 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Allium ; Fusicoccin ; Guard cell protoplasts ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Guard cell protoplasts from starch-containing Vicia faba and starch-deficient Allium cepa stomata were isolated, stabilized and recovered with an efficiency — in relation to the potential yield — of approx. 62% and 77%, respectively. In vitro, guard cell protoplasts (GCP) respond to abscisic acid and fusicoccin by respectively contracting and swelling, that is, decreasing or increasing in diameter by about 15% and more in comparison to the control. This in vitro response correlates with, but is more than 4 times as rapid as, the in vivo response of the stomata. Among the advantages presented by working with isolated GCPs are: greater sensitivity in response; freedom from influences of cuticular ridges, cell walls, subsidiary cells, and epidermal cells; and direct and parallel comparisons of starch-containing and starch-deficient GCP systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389049
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of Cl- upon the sensitivity of starch-containing and starch-deficient stomata and guard cell protoplasts towards potassium ions, fusicoccin and abscisic acid (1978)
    Springer
    Planta 144 (1978), S. 95-100 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Allium ; Chloride ; Fusicoccin ; Malate ; Stomata ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloride ions are necessary to compensate for the positively charged potassium ions imported into guard cells of Allium cepa L. during stomatal opening. Therefore an external Cl- supply of intact Allium plants is important. But high levels of chloride have been found to reduce the sensitivity of the starch-lacking stomata and isolated guard cell protoplasts (GCPs) from Allium to potassium ions, fusicoccin and abscisic acid. Furthermore, with high levels of chloride, malate anions disappear from the guard cells of Allium, a finding which contrasts with situation in Vicia where the stomatal sensitivity to K+ ions, fusicoccin and ABA is not influenced by Cl- ions and malate levels are unaffected. It is suggested that the absence of malate as a proton yielding primer inhibits the mechanism of H+/K+ exchange in Allium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385013
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