ISSN:
0886-1544
Keywords:
Tetrahymena
;
cell model
;
ATP concentration
;
Ca sensitivity
;
backward swimming
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Using Tetrahymena pyriformis, strain w, and Tetrahymena thermophila, B-1868, we prepared cell models that showed ciliary reversal with change in Ca-ion concentration, as was also noted for the Paramecium cell model. No differences could be found between these two strains in the reactivation state, and their response to environmental conditions was essentially the same. The reactivation rate was 90% or more. Swimming velocity of the cell model was found to be 200 ± 49 μm/sec at 25.0°C ± 0.5°C, while the velocity for the living cells was 527 ± 101 μm/sec. Swimming velocity with change in environmental conditions, such as pH, Mg-ATP, and Ca-ion concentrations, was studied. Compared to the cell model of Paramecium, the Tetrahymena cell model had higher sensitivity toward Ca-ion in the reactivation medium. The effects of chlorpromazine, and inhibitor of calmodulin, on the swimming behavior of the cell models were studied.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cm.970050405
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