ISSN:
1572-9818
Keywords:
Arabidopsis thaliana
;
ATHB-1
;
microprojectile-mediated gene transfer
;
transcription factors
;
transient assay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have modified the biolistic process to establish a transient assay for rapid analysis of transcription factors in arabidopsis. ATHB-1, an arabidopsis HD-Zip protein, was used as a transacting factor in particle bombardment experiments with arabidopsis leaves, and was found to transactivate a reporter gene in a binding site-specific manner. The experiments show the feasibility of using microprojectile-mediated gene transfer for rapid functional analysis of transcription factors in arabidopsis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007442607645
Permalink