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  • ASTROPHYSICS  (773)
  • Cell & Developmental Biology  (271)
  • 1980-1984  (1,024)
  • 1935-1939  (20)
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 4 (1981), S. 203-217 
    ISSN: 0148-7280
    Keywords: α-chlorohydrin ; antifertility agent ; ram ; sperm metabolism ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effects of the male antifertility agent, α-chlorohydrin, six of its derivatives, and glycidol were studied on the metabolism of washed ram spermatozoa in vitro with fructose as substrate. The α-chlorohydrin derivatives were the amino, the phosphorylated, and four glycol-bridge (ketal) compounds. All compounds except glycidol, in a concentration between 0.1 and 100 mM, reduced the aerobic glycolsis and/or oxidation of fructose. However, there was not a high correlation between the ability of these compounds to inhibit the metabolism of ram spermatozoa in vitro and their antifertility activity when administered to male rats. Other factors are clearly involved in their antifertility activity, eg, the concentration of the compounds in the epididymis and their conversion of either more or less spermicidal compounds in the body.
    Additional Material: 1 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 10-18 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active process in this system and can be triggered by K+ ions. A variety of agents which affect gene expression in cells were tested for an effect on switch-on in ISVP. Marked effects on switch-on in ISVP were observed with a diverse group of test agents, including DMSO and other solvents, BUdR, TdR, caffeine, theophylline, and temperature. The correlation in response between ISVP and cells suggests that the ISVP system may be useful as a model for studying the biochemical mechanisms underlying the perturbative effects of such agents on gene expression in cells.
    Additional Material: 14 Ill.
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  • 3
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
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  • 4
    Publication Date: 2019-07-13
    Keywords: ASTROPHYSICS
    Type: Extragalactic radio sources; Aug 03, 1981 - Aug 07, 1981; Albuquerque, NM
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  • 5
    Publication Date: 2011-08-18
    Description: NGC 7027 was observed with a multichannel grating spectrometer from 5.2 to 7.5 microns at a spectral resolving power of 120-200. Two new dust emission features are resolved at 5.62 and 6.95 microns, and for the first time the Ni II fine-structure line at 6.64 microns is detected. It is shown that a single molecular dust constituent might account for six of the nine observed dust features between 2 and 14 microns. The strength of the Ni II line indicates either that Ni is not depleted in the neutral gas, or that the line is formed at high density.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 1 (ISSN 0004-637X); 274; 666-670
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  • 6
    Publication Date: 2011-08-18
    Description: Accretion models of planet formation and the early cratering history of the solar system suggest that planet formation is accompanied by a cloud of debris resulting from accumulation and fragmentation. A rough estimate of the infrared luminosities of debris clouds is presented for comparison with measured 10-micron luminosities of young stars. New measurements of 13 F, G, and K main-sequence stars of the Ursa Major Stream, which is thought to be about 270-million years old, place constraints on the amount of debris which could be present near these stars.
    Keywords: ASTROPHYSICS
    Type: Icarus; 50; Apr. 198
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  • 7
    Publication Date: 2011-08-18
    Description: Measurements of forbidden line Ar II 6.99 micron and Pf alpha 7.45 micron made from the Kuiper Airborne Observatory are presented for Sgr A, the H II region at the center of the Galaxy. These line strengths, when combined with ground-based measurements, suggest a factor of enhancement of two in the Ar/H ratio in the galactic center region relative to that in the sun and in the solar neighborhood. The accuracy of the determination is presently limited by the uncertainty in the collision strength for Ar(+).
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal; vol. 248
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  • 8
    Publication Date: 2011-08-18
    Description: Infrared spectrophotometry of the R-type carbon star HD 19557 is presented. Two unusual spectroscopic features are seen: a 3.1 micron band is lacking and a 2.8 micron band is present. Identifications are proposed for three previously unreported stellar absorption bands with electronic sequences of C2, CN, and C2H. The latter is proposed to be responsible for the 2.8 micron feature. The atmospheric structure of the star is studied with synthetic spectra, and an effective temperature between 2600 K and 3000 K is suggested. No SiC emission is seen at 11.3 microns, indicating that grain formation is not a viable process around the star. The lack of dust in R stars may suggest a salient difference between R and N types.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 1 (ISSN 0004-637X); 270; July 1
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  • 9
    Publication Date: 2011-08-18
    Description: The importance of coordinating different types of observations of a comet is illustrated in terms of spectral data on Comet Bowell. Photometric data in 1980 indicated that OH emissions were occurring when the comet was still more than 4 AU from the sun. Further measurements were arranged with the IUE satellite, the photoelectric scanner at Cerro Tololo and the IR telescope on Mauna Kea. Several sizes of diaphragms were used in order to estimate variations with respect to aperture. Calculations were made to quantify the wavelengths observed as a function of the grains' cross-sections. The OH emission was confirmed by the spectrographic and photometric data. It is suggested that sufficient dark materials are present on the cometary surface to have enhanced the absorption of solar radiation to levels sufficient for heating and dissociating surface ice particles.
    Keywords: ASTROPHYSICS
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  • 10
    Publication Date: 2019-06-28
    Description: Measurements of forbidden Ne II lines at 12.8 microns and forbidden S IV at 10.5 microns are presented along a line through and perpendicular to the ionization front in M17 SW. These observations give evidence for ionization stratification within the front, with the most highly ionized material lying toward the east, opposite to the peak molecular emission and far-IR flux. This result supports the picture of M17 SW as a region where a molecular cloud is heated externally. The ionization equilibrium within the ridge and the nebular energetics suggest that the M17 H II region is largely excited by one or more nearby early O stars. The properties of the recently discovered radio point source in M17 are discussed.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 1 (ISSN 0004-637X); 275; 130-134
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