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  • AFLP markers  (1)
  • Anthracnose DNA fingerprinting  (1)
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    QTLs and epistasis for aluminum tolerance in rice (Oryza sativa L.) at different seedling stages (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1295-1303 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; AFLP markers ; RFLP markers ; Aluminum tolerance ; QTLs ; Epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To investigate the genetic background for aluminum (Al) tolerance in rice, a recombinant inbred (RI) population, derived from a cross between an Al-sensitive lowland indica rice variety IR1552 and an Al-tolerant upland japonica rice variety Azucena, was used in culture solution. A molecular linkage map, together with 104 amplified fragment length polymorphism (AFLP) markers and 103 restriction fragment length polymorphism (RFLP) markers, was constructed to map quantitative trait loci (QTLs) and epistatic loci for Al tolerance based on the segregation for relative root length (RRL) in the population. RRL was measured after stress for 2 and 4 weeks at a concentration of 1mM of Al3+ and a control with a pH 4.0, respectively. Two QTLs were detected at both the 2nd and the 4th weeks on chromosomes 1 and 12 from unconditional mapping, while the QTL on chromosome 1 was only detected at the 2nd stress week from conditional mapping. The effect of the QTL on chromosome 12 was increased with an increase of the stress period from 2 to 4 weeks. The QTL on chromosome 1 was expressed only at the earlier stress, but its contribution to tolerance was prolonged during growth. At least one different QTL was detected at the different stress periods. Mean comparisons between marker genotypic classes indicated that the positive alleles at the QTLs were from the Al-tolerant upland rice Azucena. An important heterozygous non-allelic interaction on Al tolerance was found. The results indicated that tolerance in the younger seedlings was predominantly controlled by an additive effect, while an epistatic effect was more important to the tolerance in older seedlings; additionally the detected QTLs may be multiple allelic loci for Al tolerance and phosphorus-uptake efficiency, or for Al and Fe2+ tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051438
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  • 2
    Electronic Resource
    Electronic Resource
    CgT1: a non-LTR retrotransposon with restricted distribution in the fungal phytopathogenColletotrichum gleosporioides (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 320-331 
    Details
    ISSN: 1617-4623
    Keywords: Retroposon ; Colletotrichum gloeosporioides ; Anthracnose DNA fingerprinting ; Horizontal transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two genetically distinct biotypes (A and B) ofColletotrichum gloeosporioides that cause different anthracnose diseases on the legumesStylosanthes spp. have been identified in Australia. A DNA sequence that was present in biotype B and absent in biotype A was isolated by differential hybridisation of a genomic library using total genomic DNA of each biotype as hybridisation probes. This sequence also failed to hybridise to DNA of three biotypes ofC. gloeosporioides from other host species and to DNA of three other species ofColletotrichum. This clone was used to isolate two cosmid clones of biotype B. Sequence analysis of these clones revealed a repetitive element of approximately 5.7 kb in length. This element, termedCgT1, was dispersed in the genome and present in about 30 copies. The element contained open reading frames encoding deduced sequence motifs homologous togag-like proteins, reverse transcriptase and RNase H domains of non-LTR retrotransposons. The termini ofCgT1 lacked long terminal repeats (LTRs) but contained a 3′ A-rich domain. The insertion site of one copy of the element was flanked by short 13-bp direct repeats. These characteristics of the termini, taken together with the overall structure and sequence homologies, indicate thatCgT1 belongs to the non-LTR, LINE-like retrotransposon class of elements that are present in many eukaryotes. PCR primers designed to amplify regions ofCgT1 can be used to distinguish biotypes A and B in Australia. DNA fingerprinting analysis of genomic DNA using hybridisation probes derived from the terminal regions ofCgT1 revealed that Australian isolates of biotype B are monomorphic.CgT1 was not detected in some isolates causing Type B disease from other countries and whenCgT1 was present there was considerable polymorphism inCgT1 organisation in the genome.CgT1 is the first transposon-like element to be identified in the genusColletotrichum and has considerable potential as a tool for the study of population structure, genome dynamics and evolution inC. gloeosporioides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173778
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    Paper (German National Licenses)
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