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  • 78.47. + p  (1)
  • DNA binding  (1)
  • 1995-1999  (2)
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  • 1995-1999  (2)
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  • 1
    ISSN: 1617-4623
    Keywords: Key wordsAspergillus ; CCAAT ; HapC ; Hap complex ; DNA binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Aspergillus nidulans hapC gene was expressed as a fusion protein with MalE or glutathione-S-transferase (GST) in Escherichia coli, and used for the purification of HapC and the preparation of anti-HapC antiserum. The CCAAT-binding factor AnCP/AnCF contains a component with an approximate molecular mass of 32 kDa that cross-reacts with the antibody. The MalE-HapC fusion protein was able to replace authentic HapC in AnCP when incubated under appropriate conditions. Furthermore, reconstitution experiments with recombinant HapC, yHAP2 and yHAP5 polypeptides showed that all three polypeptides were required for the assembly of a complex capable of binding to CCAAT-containing taaG2 promoter DNA. The relationship between AnCP/AnCF and the Saccharomyces cerevisiae HAP complex is discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 63 (1996), S. 255-264 
    ISSN: 1432-0649
    Keywords: 07.60.Ly ; 42.65.Re ; 78.47. + p
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A femtosecond frequency-domain interferometer is applied to metal-free and vanadyl phthalocyanine (H2Pc and VOPc) thin films to measure time-resolved difference phase and transmission spectra simultaneously. For both samples, the phase-change dynamics is different from the transmission-change dynamics at 620 nm, reflecting that the phthalocyanines (Pc) cannot be modeled with a two-level system but by a multi-level or inhomogeneously broadened system, in which each level pair exhibits different relaxation dynamics. Because of this dynamical difference, a phase-change measurement is required to correct distortion of the transient spectra due to induced phase modulation of probe pulses. Near zero time delay, the phase and transmission changes show different growth behavior. This behavior is explained by antisymmetric amplitude and phase gratings which are produced by coherent coupling between frequency-chirped pump and probe pulses.
    Type of Medium: Electronic Resource
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