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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European biophysics journal 14 (1987), S. 329-336 
    ISSN: 1432-1017
    Keywords: Cytochrome P-450 ; photoreversibility ; relaxational enzyme kinetics ; 7-ethoxycoumarin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract We estimate the “active” part of cytochrome P-450, which is involved in a special substrate transformation, by measuring the initial change of the production rate as a function of the relaxation transitions between two different steady states of the reaction cycle of cytochrome P-450 using the light-reversibility of the carbon monoxide inhibition. The kinetic data of such relaxations are interpreted within a model cycle, which reduces the reaction cycle to three steps. The estimation of the rate constant of the first reduction step, derived from model simulation of the production rate, is confirmed by independent experimental study of the reduction kinetics. An application of our model to the O-deethylation of 7-ethoxycoumarin reveals that — in a time average — 10%–15% of the spectroscopically detectable cytochrome P-450 is involved in that transformation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 334 (1989), S. 491-510 
    ISSN: 1434-601X
    Keywords: 25.55 ; 97.10
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The22Ne(α, γ)26Mg and22Ne(α, n)25Mg reactions were investigated forE α(lab) from 0.71 to 2.25 MeV. Neon gas enriched to 99% in22Ne was recirculated in a differentially pumped gas target system of the extended type. Theγ-ray transitions were observed with Ge(Li) detectors and the neutrons with3He ionization chambers. A previously known resonance at ER(lab)=2.05 MeV was verified and 15 new resonances were found in the energy range covered, with the lowest at ER(lab)=0.83 MeV. Information on resonance energies, widths, strengths,γ-ray branching ratios, as well asJ π assignments, is reported. The energy range investigated corresponds to the important temperature range ofT 9 from 0.3 to 1.4 (109 K), for which the astrophysical rates were determined for both reactions. The results show that the ratios of the rates for22Ne(α, n)25Mg and22Ne(α, γ)26Mg are significantly smaller than the previously adopted values, e.g., by at least a factor of 60 nearT 9=0.65. Thus, the22Ne(α, n)25Mg reaction will likely play a smaller role as a neutron source fors-process nucleosynthesis, than has frequently been assumed.
    Type of Medium: Electronic Resource
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