ISSN:
1573-4927
Keywords:
Drosophila melanogaster
;
6-phosphogluconate dehydrogenase
;
isozyme
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract 6-Phosphogluconate dehyrogenase is evident at all developmental stages of Drosophila melanogaster. The activity level is highest in early third instar larvae and declines to a lower, but relatively constant, level at all later stages of development. The enzyme is localized in the cytosolic portion of the cell. The A-isozymic form of 6-phosphogluconate dehydrogenase was purified to homogeneity and has a molecular weight of 105,000. The enzyme is a dimer consisting of subunits with molecular weights of 55,000 and 53,000. For the oxidative decarboxylation of 6-phosphogluconate the Km for substrate is 81 µm while that for NADP+ is 22.3 µm. The optimum pH for activity is 7.8 while the optimum temperature is 37 C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00504362
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