ISSN:
1432-2048
Keywords:
Anabaena (herbicide tolerance)
;
Cyanobacterium
;
5-Enolpyruvylshikimate 3-phosphate synthase
;
Glyphosate
;
Herbicide tolerance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract 5-Enolpyruvylshikimate 3-phosphate (EPSP) synthase (3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.9) from the glyphosate-tolerant cyanobacterium Anabaena variabilis (ATCC 29413) was purified to homogeneity. The enzyme had a similar relative molecular mass to other EPSP synthases and showed similar kinetic properties except for a greatly elevated K i for the herbicide glyphosate (approximately ten times higher than that of enzymes from other sources). With whole cells, the monoisopropylamine salt of glyphosate was more toxic than the free acid but the effects of the free acid and monoisopropylamine salt on purified EPSP synthase were identical.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00197039
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