ISSN:
1432-2242
Keywords:
Amitrole
;
3-amino-1,2,4-triazole
;
Cell culture
;
Herbicide tolerance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Thirty-one clones capable of growth in the presence of 1.9×10−4 M amitrole (3-amino-1,2,4-triazole) were isolated from non-mutagenized cell suspensions of haploid Nicotiana tabacum cv. ‘Wisconsin 38’ plants at a frequency of 2.5×10−8. Seven clones retained tolerance when grown on selective medium for three years. When clones were cultured in the absence of amitrole, tolerance persisted for 9 months in five clones. Some plants regenerated from three amitrole-tolerant clones were tolerant. Seven amitrole-tolerant clones were isolated from diploid N. tabacum cell suspensions and R plant tolerance was followed through two sexual generations. Simple Mendelian inheritance patterns were not observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00263406
Permalink