ISSN:
1432-203X
Keywords:
Key wordsAgrobacterium
;
Kiwifruit
;
β-1
;
3-Endo-glucanase
;
Transformation
;
Disease tolerance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Kiwifruit was transformed with a soybean β-1,3-endoglucanase (EC 3.2.1.39) cDNA under the control of the cauliflower mosaic virus (CaMV) 35S RNA promoter. The introduced gene was expressed in young leaves of the transformants. Assays of protein extracts from young leaves showed an increase in enzyme activity in many transformants, the transformant with the highest level of enzyme activity having an about sixfold increase over the control plants. When leaves from control and three transformants were inoculated with Botrytis cinerea, which causes gray mold disease, the disease lesion areas for two transformants were smaller than on control plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050616
Permalink
