ISSN:
1573-5001
Keywords:
Hydrogen exchange
;
NOESY
;
ROESY
;
Water
;
Protein
;
2D NMR
;
Calcineurin B
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A rapid and sensitive 2D approach is presented for measuring amide proton exchange rates and the NOE interaction between amide protons and water. The approach is applicable to uniformly 13C/15N-enriched proteins and can measure magnetization exchange rates in the 0.02 to 〉20s−1 range. The experiments rely on selective excitation of the water resonance, coupled with purging of underlying Hα resonances, followed by NOESY-or ROESY-type transfer to amide protons, which are dispersed by the amide 15N frequencies in an HSQC-type experiment. Two separate but interleaved experiments, with and without selective inversion of the H2O resonance, yield quantitative results. The method is demonstrated for a sample of the calcium-binding protein calcineurin B. Results indicate rapid amide exchange for the five calcineurin B residues that are analogous to the five rapidly exchanging residues in the ‘central helix’ of the homologous protein calmodulin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00198368
Permalink
