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  • 1
    Electronic Resource
    Electronic Resource
    Major differences between the rrnA operons of two strains of Agrobacterium vitis (1996)
    Springer
    Archives of microbiology 166 (1996), S. 68-70 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsAgrobacterium vitis ; Ribosomal RNA ; 16S ; rRNA ; 23S rRNA ; Bacterial phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sequence of the rrnA operon and its flanking regions was determined for the Agrobacterium vitis type strain NCPPB3554. Compared to the earlier obtained rrnA sequence of A. vitis strain S4, several important differences were noted: the sequences diverged at the 5′-flanking region, within the 16S–23S intergenic region, and within the 23S rRNA sequence. The B8 stem-loop structure at the 5′-end of the 23S rRNA of strain NCPPB3554 was 142 nt shorter than that of strain S4. These findings have important consequences for the use of ribosomal RNA gene sequences in phylogenetic comparisons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050357
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  • 2
    Electronic Resource
    Electronic Resource
    Sequence and characterisation of a ribosomal RNA operon fromAgrobacterium vitis (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 99-107 
    Details
    ISSN: 1617-4623
    Keywords: Ribosomal genes ; Agrobacterium ; Evolution ; 23S rRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract One of the four ribosomal RNA operons (rrnA) from theAgrobacterium vitis vitopine strain S4 was sequenced.rrnA is most closely related to therrn operons ofBradyrhizobium japonicum andRhodobacter sphaeroides and carries an fMet-tRNA gene downstream of its 5S gene, as in the case ofR. sphaeroides. The 16S rRNA sequence of S4 differs from theA. vitis K309 type strain sequence by only one nucleotide, in spite of the fact that S4 and K309 have very different Ti plasmids. The predicted secondary structure of the S4 23S rRNA shows several features that are specific for the alpha proteobacteria, and an unusual branched structure in the universal B8 stem. The 3′ ends of the three otherrrn copies of S4 were also cloned and sequenced. Sequence comparison delimits the 3′ ends of the four repeats and defines two groups:rrnA/rrnB andrrnC/rrnD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174350
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Sequence and characterisation of a ribosomal RNA operon from Agrobacterium vitis (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 99-107 
    Details
    ISSN: 1617-4623
    Keywords: Key words Ribosomal genes ; Agrobacterium ; Evolution ; 23S rRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  One of the four ribosomal RNA operons (rrnA) from the Agrobacterium vitis vitopine strain S4 was sequenced. rrnA is most closely related to the rrn operons of Bradyrhizobium japonicum and Rhodobacter sphaeroides and carries an fMet-tRNA gene downstream of its 5S gene, as in the case of R. sphaeroides. The 16S rRNA sequence of S4 differs from the A. vitis K309 type strain sequence by only one nucleotide, in spite of the fact that S4 and K309 have very different Ti plasmids. The predicted secondary structure of the S4 23S rRNA shows several features that are specific for the alpha proteobacteria, and an unusual branched structure in the universal B8 stem. The 3′ ends of the three other rrn copies of S4 were also cloned and sequenced. Sequence comparison delimits the 3′ ends of the four repeats and defines two groups: rrnA/rrnB and rrnC/rrnD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174350
    Location Call Number Expected Availability
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    Paper (German National Licenses)
    Fulltext
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