ISSN:
1573-6822
Keywords:
singlet oxygen
;
7,8-dihydro-8-oxo-2′-deoxyguanosine
;
DNA damage
;
8-oxo-dG
;
sodium azide
;
2,2,6,6-tetramethyl-4-piperadone
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The photogenotoxicity mechanism of quinolone antibiotics was investigated by measuring oxidative DNA damage in lomefloxacin- and UVA-exposed cultured liver-derived cells. The combination of lomefloxacin and UVA irradiation produced a dose-dependent increase in 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxo-dG) in cell DNA. This DNA damage was substantially inhibited by co-incubation with sodium azide (NaN3) or 2,2,6,6-tetramethyl-4-piperadone (TMP), chemicals that specifically quench singlet oxygen. No significant reduction of 8-oxo-dG formation was produced by N-t-butyl-α-phenylnitrone (TBP) or α-tocopherol, which primarily scavenge hydroxyl radicals. We conclude that the photodynamic generation of 8-oxo-dG by quinolones is mediated, at least in part, by singlet oxgen.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007474912498
Permalink
