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  • 12-methyl-Tetradecanoic acid; 12-methyl-Tetradecanoic acid, δ13C; 13-methyl-Tetradecanoic acid; 13-methyl-Tetradecanoic acid, δ13C; Abietic acid; Abietic acid, δ13C; Archaeol; Archaeol, δ13C; Bishomohopanoic acid; Bishomohopanoic acid, δ13C; Bishomohopanol; Bishomohopanol, δ13C; Cholesterol; Cholesterol, δ13C; cy-fatty acids C17:0w5,6; cy-fatty acids C17:0w5,6, δ13C; DEPTH, sediment/rock; Dialkyl glycerol ether C30:0; Dialkyl glycerol ether C30:0, δ13C; Diplopterol; Diplopterol, δ13C; Event label; fatty acid C16:1w5cis+trans; fatty acid C16:1w7cis+trans; fatty acid C16:1w7trans; fatty acid C17:1w6; fatty acid C17:1w8; fatty acid C18:1w7; fatty acid C18:1w9; GC; GC38; GC46; Gravity corer; Hydroxyarchaeols, extended; Hydroxyarchaeols, extended, δ13C; IFM-GEOMAR; iso-Alcohol C19, δ13C; iso-fatty acid C17:1a; iso-fatty acid C17:1a, δ13C; iso-fatty acid C17:1b; iso-fatty acid C17:1b, δ13C; Latitude of event; Leibniz-Institut für Meereswissenschaften, Kiel; Longitude of event; Mediterranean Sea; Monoalkyl glycerol ether C16:0; Monoalkyl glycerol ether C16:0, δ13C; n-fatty acid C14:0; n-fatty acid C14:0, δ13C; n-fatty acid C15:0; n-fatty acid C15:0, δ13C; n-fatty acid C16:0; n-fatty acid C16:0, δ13C; n-fatty acid C16:1w5cis+trans, δ13C; n-fatty acid C16:1w7cis+trans, δ13C; n-fatty acid C16:1w7trans, δ13C; n-fatty acid C17:1w6, δ13C; n-fatty acid C17:1w8, δ13C; n-fatty acid C18:0; n-fatty acid C18:0, δ13C; n-fatty acid C18:1w7, δ13C; n-fatty acid C18:1w9, δ13C; n-fatty acid C26:0; n-fatty acid C26:0, δ13C; n-fatty acid C28:0; n-fatty acid C28:0, δ13C; POS362/2; POS362-2_38; POS362-2_46; Poseidon; sn2-Hydroxyarchaeol; sn2-Hydroxyarchaeol, δ13C; sn3-Hydroxyarchaeol; sn3-Hydroxyarchaeol, δ13C; Tetrahymanol; Tetrahymanol, δ13C  (1)
  • FISH–secondary ion MS
Collection
Keywords
Years
  • 1
    Publication Date: 2022-05-25
    Description: Author Posting. © National Academy of Sciences, 2006. This article is posted here by permission of National Academy of Sciences for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences 103 (2006): 3846-3851, doi:10.1073/pnas.0600035103.
    Description: Studies of deeply buried, sedimentary microbial communities and associated biogeochemical processes during Ocean Drilling Program Leg 201 showed elevated prokaryotic cell numbers in sediment layers where methane is consumed anaerobically at the expense of sulfate. Here, we show that extractable archaeal rRNA, selecting only for active community members in these ecosystems, is dominated by sequences of uncultivated Archaea affiliated with the Marine Benthic Group B and the Miscellaneous Crenarchaeotal Group, whereas known methanotrophic Archaea are not detectable. Carbon flow reconstructions based on stable isotopic compositions of whole archaeal cells, intact archaeal membrane lipids, and other sedimentary carbon pools indicate that these Archaea assimilate sedimentary organic compounds other than methane even though methanotrophy accounts for a major fraction of carbon cycled in these ecosystems. Oxidation of methane by members of Marine Benthic Group B and the Miscellaneous Crenarchaeotal Group without assimilation of methane–carbon provides a plausible explanation. Maintenance energies of these subsurface communities appear to be orders of magnitude lower than minimum values known from laboratory observations, and ecosystem-level carbon budgets suggest that community turnover times are on the order of 100–2,000 years. Our study provides clues about the metabolic functionality of two cosmopolitan groups of uncultured Archaea.
    Description: This work was supported by Deutsche Forschungsgemeinschaft (to J.S.L., R.A., M.E., and K.-U.H. at Research Center for Ocean Margins and Grant Hi 616/4 to K.U.-H.); National Aeronautics and Space Administration Astrobiology Institute Grants NNA04CC06A (to J.E.B. and C.H.H. at Pennsylvania State University), NCC 2-1275 (to M.A.L., K.G.L., K.B.S., H.F.F., A.T., and K.-U.H. at the University of Rhode Island), and NCC 2-1054 (to M.L.S. and A.T. at the Marine Biological Laboratory); the G. Unger Vetlesen Foundation; U.S. Department of Energy Grant DE-FG02-93ER20117; and NSF Grant MCB03-48492. J.F.B. was supported by NSF Integrative Graduate Education and Research Traineeship Program Grant DGE-9972759 and a Schlanger fellowship from the Joint Oceanographic Institutions (JOI). M.A.L. was supported in part by postcruise support from JOI.
    Keywords: Anaerobic methanotrophy ; Deep biosphere ; FISH–secondary ion MS ; Intact polar lipids ; Stable carbon isotopes
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: 1901480 bytes
    Format: application/pdf
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  • 2
    Publication Date: 2023-06-27
    Keywords: 12-methyl-Tetradecanoic acid; 12-methyl-Tetradecanoic acid, δ13C; 13-methyl-Tetradecanoic acid; 13-methyl-Tetradecanoic acid, δ13C; Abietic acid; Abietic acid, δ13C; Archaeol; Archaeol, δ13C; Bishomohopanoic acid; Bishomohopanoic acid, δ13C; Bishomohopanol; Bishomohopanol, δ13C; Cholesterol; Cholesterol, δ13C; cy-fatty acids C17:0w5,6; cy-fatty acids C17:0w5,6, δ13C; DEPTH, sediment/rock; Dialkyl glycerol ether C30:0; Dialkyl glycerol ether C30:0, δ13C; Diplopterol; Diplopterol, δ13C; Event label; fatty acid C16:1w5cis+trans; fatty acid C16:1w7cis+trans; fatty acid C16:1w7trans; fatty acid C17:1w6; fatty acid C17:1w8; fatty acid C18:1w7; fatty acid C18:1w9; GC; GC38; GC46; Gravity corer; Hydroxyarchaeols, extended; Hydroxyarchaeols, extended, δ13C; IFM-GEOMAR; iso-Alcohol C19, δ13C; iso-fatty acid C17:1a; iso-fatty acid C17:1a, δ13C; iso-fatty acid C17:1b; iso-fatty acid C17:1b, δ13C; Latitude of event; Leibniz-Institut für Meereswissenschaften, Kiel; Longitude of event; Mediterranean Sea; Monoalkyl glycerol ether C16:0; Monoalkyl glycerol ether C16:0, δ13C; n-fatty acid C14:0; n-fatty acid C14:0, δ13C; n-fatty acid C15:0; n-fatty acid C15:0, δ13C; n-fatty acid C16:0; n-fatty acid C16:0, δ13C; n-fatty acid C16:1w5cis+trans, δ13C; n-fatty acid C16:1w7cis+trans, δ13C; n-fatty acid C16:1w7trans, δ13C; n-fatty acid C17:1w6, δ13C; n-fatty acid C17:1w8, δ13C; n-fatty acid C18:0; n-fatty acid C18:0, δ13C; n-fatty acid C18:1w7, δ13C; n-fatty acid C18:1w9, δ13C; n-fatty acid C26:0; n-fatty acid C26:0, δ13C; n-fatty acid C28:0; n-fatty acid C28:0, δ13C; POS362/2; POS362-2_38; POS362-2_46; Poseidon; sn2-Hydroxyarchaeol; sn2-Hydroxyarchaeol, δ13C; sn3-Hydroxyarchaeol; sn3-Hydroxyarchaeol, δ13C; Tetrahymanol; Tetrahymanol, δ13C
    Type: Dataset
    Format: text/tab-separated-values, 363 data points
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