ISSN:
1432-072X
Keywords:
Key wordsPseudomonas
;
1-Naphthoic acid
;
Hydrocarbon degradation
;
Hydroxylation
;
Ring
;
cleavage
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mass spectrometry. The involvement of various metabolites as intermediates in the pathway was established by demonstrating relevant enzyme activities in cell-free extracts, oxygen uptake and transformation of metabolites by the whole cells. The results obtained from such studies suggest that the degradation of 1-naphthoic acid is initiated by double hydroxylation of the aromatic ring adjacent to the one bearing the carboxyl group, resulting in the formation of 1,2-dihydroxy-8-carboxynaphthalene. The resultant diol was oxidized via 3-formyl salicylate, 2-hydroxyisophthalate, salicylate and catechol to TCA cycle intermediates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00262202
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