ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1,2-Thiazetidine 1,1-dioxides  (2)
  • Flower-specific cDNAs  (2)
  • 1
    Electronic Resource
    Electronic Resource
    The proteins encoded by two tapetum-specific transcripts, Satap35 and Satap44, from Sinipis alba L. are localized in the exine cell wall layer of developing microspores (1994)
    Springer
    Planta 192 (1994), S. 221-231 
    Details
    ISSN: 1432-2048
    Keywords: Exine ; Flower-specific cDNAs ; Maltose-binding protein fusions ; Peritapetal membrane ; Pro-Ubisch bodies ; Signal sequence ; Sinapis ; Tapetum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By differential screening of a copy DNA (cDNA) library from flowering Sinapis alba L. apices against cDNAs from vegetative apices, two cDNA clones were isolated representing transcripts that are expressed transiently at an early stage of tapetum development. The Satap35 cDNA encodes a polypeptide with a predicted molecular weight of 12.7 kDa and an isoelectric point of 10.4. The Satap44 cDNA codes for a putative 12.4-kDa polypeptide with an isoelectric point of 7.5. The deduced amino-acid sequences display 76% sequence identity and contain an N-terminal stretch of hydrophobic amino acids which has characteristics of secretory signal sequences. In-vitro transcription of the cDNAs and translation of the resulting RNAs in the presence of canine pancreatic microsomes demonstrates that the two proteins are translocated into the microsomes and that the putative preproteins are proteolytically processed to the mature forms. By immunoelectron microscopy the SaTAP35 and SaTAP44 proteins were detected at the developing peritapetal membrane between the tapetal cytoplasm and the adjacent middle layer of the anther wall. Furthermore, labelling was observed within the locule in association with globules resembling pro-Ubisch bodies which appeared at the tetrad stage. During the early vacuolate stage of microspore development the young exine was strongly labelled. The exine and the peritapetal membrane both are composed of sporopollenin, and the pro-Ubisch bodies are thought to contain sporopollenin precursors. Thus, SaTAP35 and SaTAP44 might be involved in sporopollenin formation and/or deposition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194456
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The proteins encoded by two tapetum-specific transcripts,Satap35 andSatap44, fromSinapis alba L. are localized in the exine cell wall layer of developing microspores (1994)
    Springer
    Planta 192 (1994), S. 221-231 
    Details
    ISSN: 1432-2048
    Keywords: Exine ; Flower-specific cDNAs ; Maltose-binding protein fusions ; Peritapetal membrane ; Pro-Ubisch bodies ; Signal sequence ; Sinapis ; Tapetum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By differential screening of a copy DNA (cDNA) library from floweringSinapis alba L. apices against cDNAs from vegetative apices, two cDNA clones were isolated representing transcripts that are expressed transiently at an early stage of tapetum development. TheSatap35 cDNA encodes a polypeptide with a predicted molecular weight of 12.7 kDa and an isoelectric point of 10.4. TheSatap44 cDNA codes for a putative 12.4-kDa polypeptide with an isoelectric point of 7.5. The deduced amino-acid sequences display 76% sequence identity and contain an N-terminal stretch of hydrophobic amino acids which has characteristics of secretory signal sequences. In-vitro transcription of the cDNAs and translation of the resulting RNAs in the presence of canine pancreatic microsomes demonstrates that the two proteins are translocated into the microsomes and that the putative preproteins are proteolytically processed to the mature forms. By immunoelectron microscopy theSaTAP35 andSaTAP44 proteins were detected at the developing peritapetal membrane between the tapetal cytoplasm and the adjacent middle layer of the anther wall. Furthermore, labelling was observed within the locule in association with globules resembling pro-Ubisch bodies which appeared at the tetrad stage. During the early vacuolate stage of microspore development the young exine was strongly labelled. The exine and the peritapetal membrane both are composed of sporopollenin, and the pro-Ubisch bodies are thought to contain sporopollenin precursors. Thus,SaTAP35 andSaTAP44 might be involved in sporopollenin formation and/or deposition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01089038
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Properties and Reactions of Substituted 1,2-Thiazetidine 1,1-Dioxides: 4-Alkylidene-β-sultams (1992)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1992 (1992), S. 687-692 
    Details
    ISSN: 0170-2041
    Keywords: β-Sultams ; 1,2-Thiazetidine 1,1-dioxides ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 4-α-Hydroxyalkylated β-sultams 1/5 are transformed into 4-alkylidene-β-sultams 4/7 by elimination and desilylation reactions. Another route to an exocyclic double bond in the β-sultam system has been found to be the Peterson olefination starting from 4 silylated β-sultams 8, 10, and 11. The elimination-reaction sequence yields stereochemically defined products with either E or Z configuration but by use of the Peterson reaction we have always obtained mixtures of diastereomers, which have to be separated. The stereochemistry of all products is elucidated by spectroscopic methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.1992199201116
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Properties and reactions of substituted 1,2-thiazetidine 1,1-dioxides: Acylation of β-sultams at C-4, reduction of 4-acyl-β-sultams, and stereochemistry of 4-(α-hydroxyalkyl)-β-sultams (1991)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1991 (1991), S. 171-178 
    Details
    ISSN: 0170-2041
    Keywords: β-Sultam, 4-acyl-, 4-(α-hydroxylalkyl)- ; 1,2-Thiazetidine 1,1-dioxides ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Acylation of N-silylated and 2,3-substituted 1,2-thiazetidine 1,1-dioxides (β-sultams) results in the formation of 4-acylated β-sultams 2 and 8. The desilylation of 2 to 3 is easily done with TBAF either on silica gel or in ethanolic solution. The acylated β-sultams are reduced by sodium borohydride yielding α-hydroxyalkyl β-sultams 11 and 12, obtained as mixtures of diastereoisomers. These are separated by CC. The stereochemistry of the acylated and of the α-hydroxylated β-sultams is elucidated by spectroscopic methods, and compared to that of the antibiotic drug thienamycin.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.199119910131
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...