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  • 1
    ISSN: 1432-1424
    Keywords: Cerebellar granule neuron ; Heloderma horridum ; Helothermine ; Potassium current ; Patch clamp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Helothermine, a recently isolated toxin from the venom of the Mexican beaded lizard Heloderma horridum horridum was tested on K+ currents of newborn rat cerebellar granule cells. In whole-cell voltageclamp experiments, cerebellar granule neurons exhibited at least two different K+ current components: a first transient component which is similar to an I A-type current, is characterized by fast activating and inactivating kinetics and blocked by 4-aminopyridine; a second component which is characterized by noninactivating kinetics, is blocked by tetraetylammonium ions and resembles the classical delayed-rectifier current. When added to the standard external solution at concentrations ranging between 0.1 and 2 μm helothermine reduced the pharmacologically isolated I A-type current component in a voltage- and dose-dependent way, with a half-maximal inhibitory concentration (IC50) of 0.52 μm. A comparison between control and nelothermine-modified peak transient currents shows a slowdown of activation and inactivation kinetics. The delayed-rectifier component inhibition was concentration dependent (IC50 = 0.86 μm) but not voltage dependent. No frequency-or use-dependent block was observed on both K+ current types. Perfusing the cells with control solution resulted in quite a complete current recovery. We conclude that helothermine acts with different affinities on two types of K+ current present in central nervous system neurons.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2020-12-15
    Description: In thismanuscript we present a new interpretation of the seismic series that accompanied eruptive activity off the coast of El Hierro, Canary Islands, during 2011–2013. We estimated temporal variations of the Gutenberg-Richter b value throughout the period of analysis, and performed high-precision relocations of the preeruptive and syneruptive seismicity using a realistic 3-D velocity model. Our results suggest that eruptive activity and the accompanying seismicity were caused by repeated injections of magma from the mantle into the lower crust. These magma pulses occurred within a small and well-defined volume resulting in the emplacement of fresh magma along the crust-mantle boundary underneath El Hierro. We analyzed the distribution of earthquake hypocenters in time and space in order to assess seismic diffusivity in the lower crust. Our results suggest that very high earthquake rates underneath El Hierro represent the response of a stable lower crust to stress perturbations with pulsatory character, linked to the injection of magma from the mantle. Magma input from depth caused large stress perturbations to propagate into the lower crust generating energetic seismic swarms. The absence of any preferential alignment in the spatial pattern of seismicity reinforces our hypothesis that stress perturbation and related seismicity, had diffusive character. We conclude that the temporal and spatial evolution of seismicity was neither tracking the path of magma migration nor it defines the boundaries of magma storage volumes such as a midcrustal sill. Our conceptual model considers pulsatorymagma injection fromthe uppermantle and its propagation along the Moho.We suggest, within this framework, that the spatial and temporal distributions of earthquake hypocenters reflect hydraulic fracturing processes associated with stress propagation due to magma movement.
    Description: Published
    Description: 7749–7770
    Description: 1T. Geodinamica e interno della Terra
    Description: JCR Journal
    Description: restricted
    Keywords: b value, relocation, Hydraulic fracturing, Magma influx ; 05. General::05.02. Data dissemination::05.02.99. General or miscellaneous
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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