Publication Date:
2008-04-05
Description:
Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a two-metal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406475/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406475/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Toor, Navtej -- Keating, Kevin S -- Taylor, Sean D -- Pyle, Anna Marie -- GM50313/GM/NIGMS NIH HHS/ -- R01 GM050313/GM/NIGMS NIH HHS/ -- T15 LM07056/LM/NLM NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2008 Apr 4;320(5872):77-82. doi: 10.1126/science.1153803.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, Bass Building, New Haven, CT 06511, USA. navtej.toor@yale.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18388288" target="_blank"〉PubMed〈/a〉
Keywords:
Allosteric Regulation
;
Bacillaceae/chemistry/*genetics
;
Base Pairing
;
Binding Sites
;
Catalysis
;
Catalytic Domain
;
Crystallography, X-Ray
;
Evolution, Molecular
;
*Introns
;
Ligands
;
Magnesium/chemistry
;
Models, Molecular
;
Nucleic Acid Conformation
;
Phylogeny
;
*RNA Splicing
;
RNA, Bacterial/*chemistry/metabolism
;
RNA, Catalytic/*chemistry/metabolism
;
Spliceosomes/chemistry/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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