Publication Date:
1990-01-05
Description:
One of the functions of U1 small nuclear ribonucleoprotein (snRNP) in the splicing reaction of pre-mRNA molecules is the recognition of the 5' splice site. U1 snRNP proteins as well as base-pair interactions between U1 snRNA and the 5' splice site are important for the formation of the snRNP-pre-mRNA complex. To determine which proteins are needed for complex formation, the ability of U1 snRNPs gradually depleted of the U1-specific proteins C, A, and 70k to bind to an RNA molecule containing a 5' splice site sequence was studied in a nitrocellulose filter binding assay. The most significant effect was always observed when protein C was removed, either alone or together with other U1-specific proteins; the binding was reduced by 50 to 60%. Complementation of protein C-deficient U1 snRNPs with purified C protein restored their 5' splice site binding activity. These data suggest that protein C may potentiate the base-pair interaction between U1 RNA and the 5' splice site.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Heinrichs, V -- Bach, M -- Winkelmann, G -- Luhrmann, R -- New York, N.Y. -- Science. 1990 Jan 5;247(4938):69-72.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut fuer Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2136774" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Chromatography, Affinity
;
Electrophoresis, Polyacrylamide Gel
;
Introns
;
*RNA Splicing
;
Ribonucleoproteins/genetics/*physiology
;
Ribonucleoproteins, Small Nuclear
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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