Publication Date:
2002-03-02
Description:
Brain-derived neurotrophic factor (BDNF) and other neurotrophins are critically involved in long-term potentiation (LTP). Previous reports point to a presynaptic site of neurotrophin action. By imaging dentate granule cells in mouse hippocampal slices, we identified BDNF-evoked Ca2+ transients in dendrites and spines, but not at presynaptic sites. Pairing a weak burst of synaptic stimulation with a brief dendritic BDNF application caused an immediate and robust induction of LTP. LTP induction required activation of postsynaptic Ca2+ channels and N-methyl-d-aspartate receptors and was prevented by the blockage of postsynaptic Ca2+ transients. Thus, our results suggest that BDNF-mediated LTP is induced postsynaptically. Our finding that dendritic spines are the exclusive synaptic sites for rapid BDNF-evoked Ca2+ signaling supports this conclusion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kovalchuk, Yury -- Hanse, Eric -- Kafitz, Karl W -- Konnerth, Arthur -- New York, N.Y. -- Science. 2002 Mar 1;295(5560):1729-34.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut fur Physiologie, Ludwig-Maximilians Universitat Munchen, 80336 Munchen, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11872844" target="_blank"〉PubMed〈/a〉
Keywords:
Action Potentials
;
Animals
;
Axons/drug effects/physiology
;
Brain-Derived Neurotrophic Factor/pharmacology/*physiology
;
Calcium/metabolism
;
Calcium Signaling
;
Dendrites/drug effects/*physiology
;
Dentate Gyrus/*cytology/drug effects/physiology
;
Excitatory Postsynaptic Potentials
;
In Vitro Techniques
;
*Long-Term Potentiation/drug effects
;
Mice
;
Mice, Inbred BALB C
;
Neurons/drug effects/physiology
;
Patch-Clamp Techniques
;
Perforant Pathway
;
Receptor, trkB/metabolism
;
Receptors, N-Methyl-D-Aspartate/metabolism
;
Synapses/drug effects/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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