Publication Date:
2003-12-20
Description:
Gene enrichment strategies offer an alternative to sequencing large and repetitive genomes such as that of maize. We report the generation and analysis of nearly 100,000 undermethylated (or methylation filtration) maize sequences. Comparison with the rice genome reveals that methylation filtration results in a more comprehensive representation of maize genes than those that result from expressed sequence tags or transposon insertion sites sequences. About 7% of the repetitive DNA is unmethylated and thus selected in our libraries, but potentially active transposons and unmethylated organelle genomes can be identified. Reverse transcription polymerase chain reaction can be used to finish the maize transcriptome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Palmer, Lance E -- Rabinowicz, Pablo D -- O'Shaughnessy, Andrew L -- Balija, Vivekanand S -- Nascimento, Lidia U -- Dike, Sujit -- de la Bastide, Melissa -- Martienssen, Robert A -- McCombie, W Richard -- 2 T32-CA09311-25/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2003 Dec 19;302(5653):2115-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14684820" target="_blank"〉PubMed〈/a〉
Keywords:
Algorithms
;
Chromosomes, Artificial, Bacterial
;
Cloning, Molecular
;
Computational Biology
;
Conserved Sequence
;
Contig Mapping
;
CpG Islands
;
*DNA Methylation
;
DNA Transposable Elements
;
DNA, Chloroplast/genetics
;
DNA, Complementary
;
DNA, Mitochondrial/genetics
;
DNA, Plant/genetics
;
Databases, Nucleic Acid
;
Escherichia coli/genetics
;
Exons
;
Expressed Sequence Tags
;
Genes, Plant
;
*Genome, Plant
;
Genomic Library
;
Oryza/genetics
;
Repetitive Sequences, Nucleic Acid
;
Retroelements
;
Reverse Transcriptase Polymerase Chain Reaction
;
Sequence Analysis, DNA/*methods
;
Zea mays/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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