Publication Date:
2008-11-15
Description:
Although in vitro observations suggest that cross-presentation of antigens is mediated primarily by CD8alpha+ dendritic cells, in vivo analysis has been hampered by the lack of systems that selectively eliminate this cell lineage. We show that deletion of the transcription factor Batf3 ablated development of CD8alpha+ dendritic cells, allowing us to examine their role in immunity in vivo. Dendritic cells from Batf3-/- mice were defective in cross-presentation, and Batf3-/- mice lacked virus-specific CD8+ T cell responses to West Nile virus. Importantly, rejection of highly immunogenic syngeneic tumors was impaired in Batf3-/- mice. These results suggest an important role for CD8alpha+ dendritic cells and cross-presentation in responses to viruses and in tumor rejection.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756611/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756611/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hildner, Kai -- Edelson, Brian T -- Purtha, Whitney E -- Diamond, Mark -- Matsushita, Hirokazu -- Kohyama, Masako -- Calderon, Boris -- Schraml, Barbara U -- Unanue, Emil R -- Diamond, Michael S -- Schreiber, Robert D -- Murphy, Theresa L -- Murphy, Kenneth M -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2008 Nov 14;322(5904):1097-100. doi: 10.1126/science.1164206.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19008445" target="_blank"〉PubMed〈/a〉
Keywords:
Adoptive Transfer
;
Animals
;
Antibodies, Viral/blood
;
Antigens, CD8/*analysis
;
Basic-Leucine Zipper Transcription Factors/deficiency/genetics/*physiology
;
CD4-Positive T-Lymphocytes/immunology
;
*Cross-Priming
;
*Cytotoxicity, Immunologic
;
Dendritic Cells/*immunology/transplantation
;
Female
;
Fibrosarcoma/immunology
;
Lymphocyte Activation
;
Male
;
Mice
;
Mice, Inbred C57BL
;
Repressor Proteins/genetics/*physiology
;
Spleen/immunology
;
T-Lymphocytes, Cytotoxic/*immunology
;
West Nile Fever/immunology
;
West Nile virus/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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