Publication Date:
2000-02-26
Description:
The Caenorhabditis elegans Bcl-2-like protein CED-9 prevents programmed cell death by antagonizing the Apaf-1-like cell-death activator CED-4. Endogenous CED-9 and CED-4 proteins localized to mitochondria in wild-type embryos, in which most cells survive. By contrast, in embryos in which cells had been induced to die, CED-4 assumed a perinuclear localization. CED-4 translocation induced by the cell-death activator EGL-1 was blocked by a gain-of-function mutation in ced-9 but was not dependent on ced-3 function, suggesting that CED-4 translocation precedes caspase activation and the execution phase of programmed cell death. Thus, a change in the subcellular localization of CED-4 may drive programmed cell death.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, F -- Hersh, B M -- Conradt, B -- Zhou, Z -- Riemer, D -- Gruenbaum, Y -- Horvitz, H R -- New York, N.Y. -- Science. 2000 Feb 25;287(5457):1485-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Biology, 68-425, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10688797" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Substitution
;
Animals
;
Animals, Genetically Modified
;
*Apoptosis
;
Apoptosis Regulatory Proteins
;
Caenorhabditis elegans/*cytology/embryology/genetics/*metabolism
;
*Caenorhabditis elegans Proteins
;
Calcium-Binding Proteins/genetics/*metabolism
;
*Caspases
;
Cysteine Endopeptidases/genetics/metabolism
;
Genes, Helminth
;
Helminth Proteins/genetics/*metabolism
;
Immunohistochemistry
;
Mitochondria/metabolism
;
Mutation
;
Nuclear Envelope/*metabolism
;
Phenotype
;
Proto-Oncogene Proteins/genetics/*metabolism
;
Proto-Oncogene Proteins c-bcl-2
;
Repressor Proteins/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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