Publication Date:
1997-02-21
Description:
Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yang, J -- Liu, X -- Bhalla, K -- Kim, C N -- Ibrado, A M -- Cai, J -- Peng, T I -- Jones, D P -- Wang, X -- New York, N.Y. -- Science. 1997 Feb 21;275(5303):1129-32.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9027314" target="_blank"〉PubMed〈/a〉
Keywords:
Apoproteins/metabolism
;
*Apoptosis
;
Caspase 3
;
*Caspases
;
Cysteine Endopeptidases/metabolism
;
Cytochrome c Group/*metabolism
;
Cytochromes c
;
Cytosol/metabolism
;
DNA Fragmentation
;
Enzyme Activation
;
Etoposide/pharmacology
;
HL-60 Cells
;
HeLa Cells
;
Humans
;
Intracellular Membranes/metabolism
;
Membrane Potentials/drug effects
;
Mitochondria/*metabolism
;
Poly(ADP-ribose) Polymerases/metabolism
;
Proto-Oncogene Proteins c-bcl-2/genetics/*metabolism
;
Staurosporine/pharmacology
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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