Publication Date:
2001-12-12
Description:
Heteromultimeric cyclic nucleotide-gated (CNG) channels play a central role in the transduction of odorant signals and subsequent adaptation. The contributions of individual subunits to native channel function in olfactory receptor neurons remain unclear. Here, we show that the targeted deletion of the mouse CNGA4 gene, which encodes a modulatory CNG subunit, results in a defect in odorant-dependent adaptation. Channels in excised membrane patches from the CNGA4 null mouse exhibited slower Ca2+-calmodulin-mediated channel desensitization. Thus, the CNGA4 subunit accelerates the Ca2+-mediated negative feedback in olfactory signaling and allows rapid adaptation in this sensory system.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885906/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885906/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Munger, S D -- Lane, A P -- Zhong, H -- Leinders-Zufall, T -- Yau, K W -- Zufall, F -- Reed, R R -- R37 EY006837/EY/NEI NIH HHS/ -- R37 EY006837-13/EY/NEI NIH HHS/ -- R37 EY006837-14/EY/NEI NIH HHS/ -- New York, N.Y. -- Science. 2001 Dec 7;294(5549):2172-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11739959" target="_blank"〉PubMed〈/a〉
Keywords:
1-Methyl-3-isobutylxanthine/pharmacology
;
*Adaptation, Physiological
;
Animals
;
Calcium/*metabolism
;
Calcium Signaling
;
Calmodulin/*metabolism
;
Cyclic AMP/metabolism
;
Cyclic Nucleotide-Gated Cation Channels
;
Cyclohexanols/pharmacology
;
Electrophysiology
;
Gene Targeting
;
Ion Channel Gating
;
Ion Channels/*genetics/*physiology
;
Kinetics
;
Mice
;
Mice, Inbred C57BL
;
*Monoterpenes
;
*Odors
;
Olfactory Bulb/physiology
;
Olfactory Mucosa/physiology
;
Olfactory Receptor Neurons/metabolism/*physiology
;
Protein Subunits
;
Terpenes/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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