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  • 1
    ISSN: 0749-503X
    Keywords: Candida albicans ; HIS4 ; complementation ; molecular biology tools ; topological marker ; amino acid biosynthesis general control ; PEX5 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have isolated the Candida albicans HIS4 (CaHIS4) gene by complementation of a his4-34 Saccharomyces cerevisiae mutant. The sequenced DNA fragment contains a putative ORF of 2514 bp, whose translation product shares a global identity of 44% and 55% to the His4 protein homologs of S. cerevisiae and Kluyveromyces lactis, respectively. Analysis of CaHIS4 sequence suggests that, similarly to S. cerevisiae HIS4, it codes for a polypeptide having three separate enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase) which reside in different domains of the protein. A C. albicans his4 strain is complemented with this gene when using a C. albicans-S. cerevisiae-Escherichia coli shuttle vector, thus enabling the construction of a host system for C. albicans genetic manipulation. In addition, upstream of the sequenced CaHIS4 sequence, we have found the 3′-terminal half of a gene encoding a PEX5-like protein. The EMBL/DDJB/GenBank Accession Number of this sequence is AJ003115. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2018-05-30
    Description: Most of articles that have recently appeared studying electronic service quality propose scales that are focused on measuring the quality of a particular service. However, could these scales be applied to measure the quality of other online services? In this situation, the main purpose of this study is to test the equivalence of a factor model to evaluate the quality of three services: online travel reservations, accommodation reservations and online ticketing. The results support the measurement equivalence of the measures. However, the importance of the dimensions varies across the services. In this sense, reliability is the most important factor in the evaluation of Websites of travel and accommodation reservations. Furthermore, in the evaluation of ticketing Websites, the higher weight factor is design.
    Keywords: ddc:650 ; electronic service quality ; measurement invariance ; multigroup ; confirmatory factor analysis ; Website
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: English
    Type: doc-type:article
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