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  • 1
    ISSN: 1432-0878
    Keywords: Muscle ultrastructure ; (Na+, K+)-ATPase localization ; Sarcotubules ; Sarcoplasmic reticulum ; Junctional SR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary ATPase activity sensitive to ouabain was examined in both cardiac (ventricular) and skeletal (tibialis anterior) muscle cells of the mouse. Short-term fixation was combined with incubation in a medium designed to reduce artifactual deposition of lead phosphate. With incubation medium containing Na+ and K+, Pb3 (PO4)2 precipitate appears throughout the sarcoplasmic reticulum (SR) of both cardiac and skeletal cells. The precipitate generally is heavier in the junctional SR than in network SR, although the two regions are interconnected. Ouabain (1 mM) eliminates activity in the network SR of myocardial cells, but only reduces it in skeletal muscle cells. The total ATPase activity of junctional cisternae of the SR of myocardial cells does not appear to be reduced by ouabain, whereas the activity of the terminal cisternae of skeletal muscle is substantially diminished. The use of an incubation medium containing zero K+ reduces the level of activity, but not consistently. These data suggest that (Na+, K+)-ATPase is present in the network SR of both cardiac and skeletal muscle cells, and probably in the terminal cisternae of skeletal muscle cells.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 200 (1979), S. 367-382 
    ISSN: 1432-0878
    Keywords: Ruthenium red ; Skeletal muscle ; Cardiac muscle ; Membrane permeability ; Sarcoplasmic reticulum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of ruthenium red (RR) on amphibian and mammalian skeletal muscles and mammalian myocardium were examined. In skeletal muscle cells, a discrete pattern of staining can be brought about within the lumina of the terminal cisternae (junctional sarcoplasmic reticulum [SR]) by sequential exposure to RR and OsO4. After prolonged immersion in RR solution, formation of pentalaminar segments (“zippering”) occurs at various points along the longitudinal (“network”) SR tubules. Zippering can be elicited in skeletal SR at any stage of preparation prior to postfixation with OsO4. By means of dispersive X-ray analysis, both ruthenium and osmium were seen to be deposited in skeletal muscle junctional SR, and ruthenium was detected in the myoplasm as well. In skeletal muscles whose T tubules were ruptured by exposure to glycerol, the pattern of SR staining and zippering resulting from ruthenium-osmium treatment was not affected. These findings indicate that RR is capable of passage across the sarcolemma of skeletal muscle and that this passage does not occur solely under conditions in which the plasma membrane is damaged. In contrast, RR does not opacify or modify any region of the SR of cardiac muscle. However, after this treatment, randomly distributed opaque bodies, composed of parallel lamellar structures, appear throughout the myocardial cells. A few of these bodies are associated with lipid droplets, but the rest are of unknown origin. The failure of the SR of cardiac muscle to stain after exposure to ruthenium dye (even though this material enters these cells) suggests that the chemical composition of cardiac SR is significantly different from that of skeletal muscle SR.
    Type of Medium: Electronic Resource
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