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  • 1
    ISSN: 1432-2048
    Keywords: Embryo (peptide carrier) ; Hordeum (embryo, peptide transport) ; Peptide transport ; Photoaffinity probe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The preparation of a phenylalanine analogue containing an azido group and its incorporation into dipeptides is described. Peptides modified in this way are taken up into barley (Hordeum vulgare L.) scutella via the previously characterized peptide-transport system. Photoactivation of modified peptides in the presence of isolated scutella resulted in irreversible inhibition of peptide uptake in a concentration-dependent manner. Transport of other solutes which share a common mechanism of energy coupling, but which are transported via distinct carriers, was not inhibited after photo-derivatization of scutella with the modified peptides. Derivatization of isolated scutellar tissue with a 14C-labelled peptide analogue, resulted in incorporation of label into two proteins of Mr = 54000 and 41000. Scutellar tissue from early-germinating seeds, which do not show active peptide uptake, did not incorporate label into these polypeptides. It is concluded that these proteins are components of the barley peptide-transport system.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2013-08-31
    Description: The results of the Trapped Radiation Effects Panel for the Space Environmental Effects on Materials Workshop are presented. The needs of the space community for new data regarding effects of the space environment on materials, including electronics are listed. A series of questions asked of each of the panels at the workshop are addressed. Areas of research which should be pursued to satisfy the requirements for better knowledge of the environment and better understanding of the effects of the energetic charged particle environment on new materials and advanced electronics technology are suggested.
    Keywords: CHEMISTRY AND MATERIALS (GENERAL)
    Type: NASA(SDIO Space Environmental Effects on Materials Workshop, Part 2; p 597-605
    Format: application/pdf
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