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  • Genetics  (3)
  • Customer satisfaction  (1)
  • M10  (1)
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 12 (1996), S. 1677-1702 
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: No Abstract
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0749-503X
    Keywords: Candida albicans ; HIS4 ; complementation ; molecular biology tools ; topological marker ; amino acid biosynthesis general control ; PEX5 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have isolated the Candida albicans HIS4 (CaHIS4) gene by complementation of a his4-34 Saccharomyces cerevisiae mutant. The sequenced DNA fragment contains a putative ORF of 2514 bp, whose translation product shares a global identity of 44% and 55% to the His4 protein homologs of S. cerevisiae and Kluyveromyces lactis, respectively. Analysis of CaHIS4 sequence suggests that, similarly to S. cerevisiae HIS4, it codes for a polypeptide having three separate enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase) which reside in different domains of the protein. A C. albicans his4 strain is complemented with this gene when using a C. albicans-S. cerevisiae-Escherichia coli shuttle vector, thus enabling the construction of a host system for C. albicans genetic manipulation. In addition, upstream of the sequenced CaHIS4 sequence, we have found the 3′-terminal half of a gene encoding a PEX5-like protein. The EMBL/DDJB/GenBank Accession Number of this sequence is AJ003115. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
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  • 3
    ISSN: 0749-503X
    Keywords: Firefly luciferase ; yeast expression ; gene reporter ; Saccharomyces cerevisiae ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The LUC gene coding for Photinus pyralis firefly luciferase was cloned in different yeast episomal plasmids in order to assess its possibilities as an in vivo reporter gene. Activity of the enzyme in transformed cells in vivo was measured by following light emission and assay conditions optimized in intact cells, with regard to oxygen concentration, temperature, cell concentration in assay mixtures and external ATP concentration. Among the factors tested, light emission was drastically influenced by the external pH in the assay (which resulted in a ten-fold amplification signal) and by substrate permeability. The growth phase of the cells was also important for the level of activity detected. Cloning of firefly luciferase gene under the control of different yeast-regulated promoters (ADH1, GAL1-10) enabled us to measure their strength which correlated well with previously described data. We conclude that firefly luciferase is an adequate gene reporter for the in vivo sensitive determination of gene expression and promoter strength in yeast.
    Additional Material: 5 Ill.
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  • 4
    Publication Date: 2019-03-14
    Description: The main objective of this study is to determine the influence of service quality on satisfaction of football spectators, looking scarcely investigated in the literature. The results, from a sample of 700 spectators at a football club of the Spanish First Division, reveal three main conclusions: (i) the quality of service of a football event is determined by both tangibles - infrastructure - as intangible - quality and delivery of equipment, and management of the board; (ii) the quality of positively affects service satisfaction experienced by the viewer part associated with football event; (iii) in overall satisfaction directly influences partial satisfaction associated with specific aspects of the sporting event and indirectly the quality of services provided. The study concludes with the main contributions, limitations and future research.
    Keywords: M31 ; ddc:330 ; Perceived quality ; Service ; Customer satisfaction ; Sport event ; Football
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: Spanish
    Type: doc-type:article
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  • 5
    Publication Date: 2018-12-19
    Description: The preoccupation to consider the heterogeneity in firm behavior within an activity sector has been one of the main reasons for the search and identification of strategic groups, generating an inexhaustible debate in the scope of the strategic management. Nevertheless, this preoccupation has not been limited to identify firm archetypes or configurations, but also it has extended to the study of possible differences in performance between the identified groups. In this paper, we tried to answer to this double problem focusing in the scope of franchising. A data base including all the franshisor chains (664) that operated in Spain in 2005 has been elaborated. Results reveal the existence of five strategic groups (types of franchisors) perfectly differentiated, which are described from the strategic variables they define them. Also, the existence of differences between groups with regard to results is shown based on some performance measures (income, ordinary results and profit).
    Keywords: M10 ; M19 ; ddc:330 ; franchising ; franchisors ; strategic groups ; performance ; profit
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: Spanish
    Type: doc-type:article
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