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  • Articles  (3)
  • Firefly luciferase  (1)
  • profit
  • franchised units
  • 1
    ISSN: 0749-503X
    Keywords: Firefly luciferase ; yeast expression ; gene reporter ; Saccharomyces cerevisiae ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The LUC gene coding for Photinus pyralis firefly luciferase was cloned in different yeast episomal plasmids in order to assess its possibilities as an in vivo reporter gene. Activity of the enzyme in transformed cells in vivo was measured by following light emission and assay conditions optimized in intact cells, with regard to oxygen concentration, temperature, cell concentration in assay mixtures and external ATP concentration. Among the factors tested, light emission was drastically influenced by the external pH in the assay (which resulted in a ten-fold amplification signal) and by substrate permeability. The growth phase of the cells was also important for the level of activity detected. Cloning of firefly luciferase gene under the control of different yeast-regulated promoters (ADH1, GAL1-10) enabled us to measure their strength which correlated well with previously described data. We conclude that firefly luciferase is an adequate gene reporter for the in vivo sensitive determination of gene expression and promoter strength in yeast.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2018-12-19
    Description: Franchising system continues its unstoppable advance worldwide, as in the most commercially developed countries as in those that have some more antiquated commercial structures. For that reason, nowadays, this is an important research topic in the business area. Franchising chains expand themselves through own and franchised units, in this context, this work intends to determine what kinds of units are more efficient. Own and franchised units are compared by the following indexes: sales per employee, sales per establishment and employees per establishment. As performances measures has been used: ROI (return on investment), incomes and ordinary results. This field of research has not been developed in Spain and scantly treated in the scientific international literature.
    Keywords: ddc:330 ; franchise system ; franchised units ; own units ; eficiency ; agency theory ; resource scarcity theory ; extension risk theory
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: Spanish
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  • 3
    Publication Date: 2018-12-19
    Description: The preoccupation to consider the heterogeneity in firm behavior within an activity sector has been one of the main reasons for the search and identification of strategic groups, generating an inexhaustible debate in the scope of the strategic management. Nevertheless, this preoccupation has not been limited to identify firm archetypes or configurations, but also it has extended to the study of possible differences in performance between the identified groups. In this paper, we tried to answer to this double problem focusing in the scope of franchising. A data base including all the franshisor chains (664) that operated in Spain in 2005 has been elaborated. Results reveal the existence of five strategic groups (types of franchisors) perfectly differentiated, which are described from the strategic variables they define them. Also, the existence of differences between groups with regard to results is shown based on some performance measures (income, ordinary results and profit).
    Keywords: M10 ; M19 ; ddc:330 ; franchising ; franchisors ; strategic groups ; performance ; profit
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: Spanish
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